GeneTex
United States (US)

COL5A3 antibody

GTX34325_20170112_WB_1_18121410_952.jpg
GTX34325_20170112_WB_18121410_141.jpg

Cat No. GTX34325

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, ELISA, IHC
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
ELISA Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

172 kDa. ( Note )

Specificity/Sensitivity

COL5A3 Polyclonal Antibody detects endogenous levels of COL5A3 protein.
PROPERTIES

Form

Liquid

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Synthesized peptide derived from the N-terminal region of human COL5A3 at AA range: 190-270.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

collagen type V alpha 3 Antibody

Background

This gene encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. Mutations in this gene are thought to be responsible for the symptoms of a subset of patients with Ehlers-Danlos syndrome type III. Messages of several sizes can be detected in northern blots but sequence information cannot confirm the identity of the shorter messages. [provided by RefSeq, Jul 2008]
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Database

DATA IMAGES
GTX34325_20170112_WB_1_18121410_952.jpg

GTX34325 WB Image

Western Blot (WB) analysis of HUVEC cells using COL5A3 Polyclonal Antibody.

GTX34325_20170112_WB_18121410_141.jpg

GTX34325 WB Image

Western Blot (WB) analysis of specific cells using COL5A3 Polyclonal Antibody.

Package List Price ($)
$ 319