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COMMD10 antibody

KO/KD Orthogonal
Anti-COMMD10 antibody used in Western Blot (WB). GTX121488
Anti-COMMD10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121488
Anti-COMMD10 antibody used in IHC (Paraffin sections) (IHC-P). GTX121488
Anti-COMMD10 antibody used in Western Blot (WB). GTX121488
Anti-COMMD10 antibody used in Western Blot (WB). GTX121488
Anti-COMMD10 antibody used in Western Blot (WB). GTX121488
Anti-COMMD10 antibody used in Western Blot (WB). GTX121488
Anti-COMMD10 antibody used in Western Blot (WB). GTX121488

Cat. No. GTX121488

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all COMMD10 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human COMMD10. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11163444

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

COMM domain containing 10 , PTD002

Database

Research Area

DATA IMAGES

Anti-COMMD10 antibody used in Western Blot (WB). GTX121488

GTX121488 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
12% SDS PAGE
GTX121488 diluted at 1:1000

Anti-COMMD10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121488

GTX121488 ICC/IF Image

COMMD10 antibody detects COMMD10 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: COMMD10 protein stained by COMMD10 antibody (GTX121488) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-COMMD10 antibody used in IHC (Paraffin sections) (IHC-P). GTX121488

GTX121488 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using COMMD10 antibody(GTX121488) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-COMMD10 antibody used in Western Blot (WB). GTX121488

GTX121488 WB Image

KO/KD

The data was published in the journal Front Physiol in 2018.PMID: 29997525

Anti-COMMD10 antibody used in Western Blot (WB). GTX121488

GTX121488 WB Image

KO/KD

The data was published in the journal Front Physiol in 2018.PMID: 29997525

Anti-COMMD10 antibody used in Western Blot (WB). GTX121488

GTX121488 WB Image

Orthogonal

The data was published in the journal Front Immunol in 2018. PMID: 30487795

Anti-COMMD10 antibody used in Western Blot (WB). GTX121488

GTX121488 WB Image

KO/KD Orthogonal

The data was published in the journal Front Immunol in 2018. PMID: 30487795

Anti-COMMD10 antibody used in Western Blot (WB). GTX121488

GTX121488 WB Image

The data was published in the journal Front Physiol in 2018.PMID: 29997525

REFERENCE

Application Reference

Healy MD et al. Cell 2023; 186 (10) : 2219-2237.e29 Structure of the endosomal Commander complex linked to Ritscher-Schinzel syndrome.
Ware AW et al. Front Physiol 2018; 9 : 793 Epithelial Na + Channel: Reciprocal Control by COMMD10 and Nedd4-2.
Mouhadeb O et al. Front Immunol 2018; 9 : 2623 Impaired COMMD10-Mediated Regulation of Ly6Chi Monocyte-Driven Inflammation Disrupts Gut Barrier Function.
Application : WB
Reactivity : Mouse
Fedoseienko A et al. Circ Res 2018; (Epub) COMMD Family Regulates Plasma LDL Levels and Attenuates Atherosclerosis Through Stabilizing the CCC Complex in Endosomal LDLR Trafficking.
Application : WB
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169