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COMMD7 antibody

KO/KD
Anti-COMMD7 antibody used in IHC (Whole mount) (IHC-Wm). GTX112076
Anti-COMMD7 antibody used in IHC (Paraffin sections) (IHC-P). GTX112076
Anti-COMMD7 antibody used in Western Blot (WB). GTX112076
Anti-COMMD7 antibody used in IHC (Paraffin sections) (IHC-P). GTX112076
Anti-COMMD7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112076

Cat. No. GTX112076

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Wm

Reactivity

 Human, Mouse, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all COMMD7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Positive Control

293T , A431 , H1299

Product Note

Knockdown/Knockout validation was supported by references (PMID:37057209)

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human COMMD7. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036630

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

COMM domain containing 7 , C20orf92 , dJ1085F17.3

Database

Research Area

DATA IMAGES

Anti-COMMD7 antibody used in IHC (Whole mount) (IHC-Wm). GTX112076

GTX112076 IHC-Wm Image

COMMD7 antibody detects Commd7 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
COMMD7 antibody (GTX112076) dilution: 1:100.
Anti-COMMD7 antibody used in IHC (Paraffin sections) (IHC-P). GTX112076

GTX112076 IHC-P Image

Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using COMMD7(GTX112076) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-COMMD7 antibody used in Western Blot (WB). GTX112076

GTX112076 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: H1299
12% SDS PAGE
GTX112076 diluted at 1:1000

Anti-COMMD7 antibody used in IHC (Paraffin sections) (IHC-P). GTX112076

GTX112076 IHC-P Image

COMMD7 antibody detects COMMD7 protein at nucleus in mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
COMMD7 antibody (GTX112076) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-COMMD7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112076

GTX112076 ICC/IF Image

COMMD7 antibody detects COMMD7 protein at nucleus and cytoplasm by immunofluorescent analysis.
Sample: SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: COMMD7 protein stained by COMMD7 antibody (GTX112076) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

Application Reference

Healy MD et al. Cell 2023; 186 (10) : 2219-2237.e29 Structure of the endosomal Commander complex linked to Ritscher-Schinzel syndrome.
Shao X et al. Int J Med Sci 2023; 20 (4) : 520-529 ZNF460-regulated COMMD7 Promotes Acute Myeloid Leukemia Proliferation Via the NF-κB Signaling Pathway.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169