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COMT ELISA Pair

COMT ELISA Pair. GTX500009

Cat No. GTX500009

Application

ELISA, Sandwich ELISA

Reactivity

Human
Package
1 pair ($399)
PRODUCT

Product Content

GTX101233
Type : Capture Antibody
Host : Rabbit
Conjugation : Unconjugated
Package : 50 μl
GTX89277
Type : Detection Antibody
Host : Goat
Conjugation : Unconjugated
Package : 50 μl

APPLICATION

Application Note

We recommend using the capture antibody at a concentration of 2.5μg/mL and the detection antibody at a concentration of 0.5μg/mL. Optimal dilutions should be determined experimentally by the researcher.

PROPERTIES

Form

Liquid

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC.  For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Catechol-O-Methyltransferase , Hel-S-98N , Comt

Background

Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters. [provided by RefSeq]

Research Area

DATA IMAGES

COMT ELISA Pair. GTX500009

GTX500009 ELISA Image

ELISA detection of COMT using GTX101233 for capture at a concentration of 2.5 μg/mL and GTX89277 for detection at a concentration of 0.5 μg/mL. The HRP-conjugated anti-goat IgG antibody (GTX26741) was diluted at 1:20,000-1:200,000 and used to detect the primary antibody.

REFERENCE

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REVIEW

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