APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.03-0.1μg/ml |
| 3.75μg/ml |
| Assay dependent |
| Assay dependent |
|
Note :
IHC-P
Human Brain (Cerebellum) shows staining of processes coming from the edge of the granular zone into the molecular layer.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89277-PEP).
Product Note
This antibody is expected to recognise both reported isoforms (NP_000745.1 and NP_009294.1)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-QDIIPQLKKKYDVD, from the internal region of the protein sequence according to NP_000745.1; NP_009294.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
catechol-O-methyltransferase , HEL-S-98n
Background
Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters. [provided by RefSeq, Sep 2008]
Database
Research Area
DATA IMAGES
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GTX89277 ELISA Image
ELISA analysis of COMT protein using GTX89277 COMT antibody, Internal.
Capture : GTX101233 (2.5μg/ml)
Detection : GTX89277 (0.5μg/ml)
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GTX89277 IHC-P Image
IHC-P analysis of human cerebellum using GTX89277 COMT antibody, Internal.
Antigen retrieval : Tris/EDTA buffer pH 9
Dilution : 4μg/ml
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GTX89277 IHC-P Image
IHC-P analysis of human small intestine using GTX89277 COMT antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml
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GTX89277 WB Image
WB analysis of human testis lysate using GTX89277 COMT antibody, Internal.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer
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GTX89277 IHC-P Image
IHC-P analysis of human testis using GTX89277 COMT antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml
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REFERENCE
There are currently no references for COMT antibody, Internal (GTX89277). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for COMT antibody, Internal (GTX89277). Be the first to share your experience with this product.
