GeneTex
  • Country / Location Selection

United States (US)

COMT antibody

Anti-COMT antibody used in Western Blot (WB). GTX101233
Anti-COMT antibody used in IHC (Paraffin sections) (IHC-P). GTX101233
Anti-COMT antibody used in Western Blot (WB). GTX101233
Anti-COMT antibody used in Western Blot (WB). GTX101233
Anti-COMT antibody used in ELISA (ELISA). GTX101233

Cat No. GTX101233

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA, sELISA
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
ELISA 1:1000-1:10000
sELISA Assay dependent

Note :

sELISA
Capture: GTX101233 , Detection: GTX89277

Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

Molt-4 , mouse spleen

Predict Reactivity

Rat, Bovine, Dog, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.32 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human COMT. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

catechol-O-methyltransferase,HEL-S-98n

Cellular Localization

Isoform Soluble: Cytoplasm , Isoform Membrane-bound: Cell membrane

Background

Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-COMT antibody used in Western Blot (WB). GTX101233

GTX101233 WB Image

Wild-type (WT) and COMT knockout (KO) HEK293T cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with COMT antibody (GTX101233) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-COMT antibody used in IHC (Paraffin sections) (IHC-P). GTX101233

GTX101233 IHC-P Image

COMT antibody detects COMT protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
COMT antibody (GTX101233) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-COMT antibody used in Western Blot (WB). GTX101233

GTX101233 WB Image

Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
12% SDS PAGE
GTX101233 diluted at 1:1000

Anti-COMT antibody used in Western Blot (WB). GTX101233

GTX101233 WB Image

COMT antibody detects COMT protein by Western blot analysis.
A. 50 µg mouse spleen lysate/extract
12 % SDS-PAGE
COMT antibody (GTX101233) dilution: 1:500

Anti-COMT antibody used in ELISA (ELISA). GTX101233

GTX101233 ELISA Image

ELISA detection of COMT using GTX101233 for capture at a concentration of 2.5 µg/mL and GTX89277 for detection at a concentration of 0.5 µg/mL.

REFERENCE
REVIEW
There are currently no reviews for COMT antibody (GTX101233). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169