APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Raji
Predict Reactivity
Mouse, Zebrafish, Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.54 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human COPB. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1950017
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
coatomer protein complex subunit beta 1 , COPB
Cellular Localization
Cytoplasm , Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side , Cytoplasmic vesicle , COPI-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side , Cytoplasm , Golgi apparatus membrane , Cytoplasmic vesicle , COPI-coa
Background
This gene encodes a protein subunit of the coatomer complex associated with non-clathrin coated vesicles. The coatomer complex, also known as the coat protein complex 1, forms in the cytoplasm and is recruited to the Golgi by activated guanosine triphosphatases. Once at the Golgi membrane, the coatomer complex may assist in the movement of protein and lipid components back to the endoplasmic reticulum. Alternatively spliced transcript variants have been described. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-COPB antibody [N1N2], N-term used in Western Blot (WB). GTX113167](/templates/default/js/lightbox/images/loading.gif "Anti-COPB antibody [N1N2], N-term used in Western Blot (WB). GTX113167")
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GTX113167 WB Image
Sample (30 ug of whole cell lysate)
A: Raji
7.5% SDS PAGE
GTX113167 diluted at 1:1000
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GTX113167 IHC-P Image
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using COPB(GTX113167) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX113167 IHC-P Image
COPB antibody [N1N2], N-term detects COPB protein at cytoplasm in rat testis by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
COPB antibody [N1N2], N-term (GTX113167) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX113167 ICC/IF Image
Immunofluorescence analysis of methanol-fixed HeLa, using COPB(GTX113167) antibody at 1:100 dilution.
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REFERENCE
There are currently no references for COPB antibody [N1N2], N-term (GTX113167). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for COPB antibody [N1N2], N-term (GTX113167). Be the first to share your experience with this product.
![Anti-COPB antibody [N1N2], N-term used in Western Blot (WB). GTX113167](/upload/website/prouct_img/small/GTX113167/GTX113167_40100_WB_w_23060500_530.webp "Anti-COPB antibody [N1N2], N-term used in Western Blot (WB). GTX113167")
![Anti-COPB antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113167](/upload/website/prouct_img/small/GTX113167/GTX113167_40100_IHC_w_23060500_826.webp "Anti-COPB antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113167")
![Anti-COPB antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113167](/upload/website/prouct_img/small/GTX113167/GTX113167_40100_20150512_IHC_R_w_23060501_190.webp "Anti-COPB antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113167")
![Anti-COPB antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113167](/upload/website/prouct_img/small/GTX113167/GTX113167_40100_IFA_w_23060501_837.webp "Anti-COPB antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113167")
![Anti-COPB antibody [N1N2], N-term used in Western Blot (WB). GTX113167](/upload/website/prouct_img/normal/GTX113167/GTX113167_40100_WB_w_23060500_530.webp "Anti-COPB antibody [N1N2], N-term used in Western Blot (WB). GTX113167")
![Anti-COPB antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113167](/upload/website/prouct_img/normal/GTX113167/GTX113167_40100_IHC_w_23060500_826.webp "Anti-COPB antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113167")
![Anti-COPB antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113167](/upload/website/prouct_img/normal/GTX113167/GTX113167_40100_20150512_IHC_R_w_23060501_190.webp "Anti-COPB antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX113167")
![Anti-COPB antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113167](/upload/website/prouct_img/normal/GTX113167/GTX113167_40100_IFA_w_23060501_837.webp "Anti-COPB antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113167")