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COPB antibody

Anti-COPB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22899
Anti-COPB antibody used in Western Blot (WB). GTX22899
Anti-COPB antibody used in Western Blot (WB). GTX22899
Anti-COPB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22899

Cat. No. GTX22899

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, FACS, IP, IHC

Reactivity

 Human, Mouse, Rat, Yeast, Bovine, Hamster, Primate
Package
100 μl ($399)
See all COPB products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 2 μg/mL
ICC/IF 2 μg/mL
FACS 3-5 μg/10⁶ cells
IP Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

107 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

4.4 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Synthetic peptide corresponding to residues E(496) A G E L K P E E E I T V G P V Q K(513) of rat beta-COP.

Purification

Ammonium sulfate precipitation

Conjugation

Unconjugated

RRID

AB_384898

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

coatomer protein complex, subunit beta 1 , 2610019B04Rik

Cellular Localization

Cytoplasm,Golgi apparatus membrane,Cell membrane

Background

Coatomer proteins are involved in regulating transport between the endoplasmic reticulum (ER) and the Golgi complex and in intra-Golgi transport. There exist two coatomer-protein mechanisms (COPI and COPII) and although they have mechanistic parallels, they are molecularly distinct. The COPI coat is comprised of seven subunits (alpha-, beta-, beta'-, gamma-, delta-, epsilon-, and zeta-COP) in a complex called coatomer. Assembly of the coatomer (COPI) onto non-clathrin coated vesicles is regulated by ADP-ribosylation factor (ARF). Vesicle formation, budding, fusion, and disassembly is dependent on GDP-GTP exchange, COPI, and ARF. COPI has been shown to facilitate retrograde intracellular transport from the ER to the Golgi complex. By contrast, COPII facilitates anterograde transport between these subcellular organelles. COPII has been shown to be independently and selectively recruited to the ER relative to COPI subunits.

Database

Research Area

DATA IMAGES

Anti-COPB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22899

GTX22899 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX22899 COPB antibody.

Anti-COPB antibody used in Western Blot (WB). GTX22899

GTX22899 WB Image

WB analysis of rat brain homogenate using GTX22899 COPB antibody.

Anti-COPB antibody used in Western Blot (WB). GTX22899

GTX22899 WB Image

WB analysis of membrane enriched extracts (30 ug) of Mouse Brain (Lane 1), NIH/3T3 (Lane 2), PC-3 (Lane 3), C2C12 (Lane 4), HeLa (Lane 5), HCT 116 (Lane 6) and COLO 205 (Lane 7) using GTX22899 COPB antibody.
Dilution : 2 μg/ml

Anti-COPB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22899

GTX22899 ICC/IF Image

ICC/IF analysis of L6 cells using GTX22899 COPB antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 2 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature

REFERENCE

Application Reference

Baron DM et al. Hum Mol Genet 2019; 28 (13) : 2143-2160 Quantitative proteomics identifies proteins that resist translational repression and become dysregulated in ALS-FUS.
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399