GeneTex
United States (US)

COPD antibody

KO/KD
GTX103252_39918_20160630_WB_shRNA_watermark_19011506_498.jpg
GTX103252_40240_20180530_IHC-Fr_M_2_19011506_324.jpg
GTX103252_40240_20180525_IHC-P_M_19011506_473.jpg
GTX103252_WB_19011506_599.jpg
GTX103252_39918_IFA_19011506_588.jpg

Cat No. GTX103252

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Raji

Predict Reactivity

Rat, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.21 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human COPD. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Archain 1,Copd,Srmmd,Arcn1

Cellular Localization

Cytoplasm , Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side , Cytoplasmic vesicle , COPI-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side , Cytoplasm , Golgi apparatus membrane , Cytoplasmic vesicle , COPI-coa

Background

This gene maps in a region, which include the mixed lineage leukemia and Friend leukemia virus integration 1 genes, where multiple disease-associated chromosome translocations occur. It is an intracellular protein. Archain sequences are well conserved among eukaryotes and this protein may play a fundamental role in eukaryotic cell biology. It has similarities to heat shock proteins and clathrin-associated proteins, and may be involved in vesicle structure or trafficking. [provided by RefSeq]

Database

DATA IMAGES
GTX103252_39918_20160630_WB_shRNA_watermark_19011506_498.jpg

GTX103252 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with COPD antibody (GTX103252) diluted at 1:2000.

GTX103252_40240_20180530_IHC-Fr_M_2_19011506_324.jpg

GTX103252 IHC-Fr Image

COPD antibody detects COPD protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse mouse cerebellum.
Green: COPD stained by COPD antibody (GTX103252) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

GTX103252_40240_20180525_IHC-P_M_19011506_473.jpg

GTX103252 IHC-P Image

COPD antibody detects COPD protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
COPD stained by COPD antibody (GTX103252) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

GTX103252_WB_19011506_599.jpg

GTX103252 WB Image

Sample(30 ug whole cell lysate)
A:H1299
B:Hep G2(GTX27900)
7.5% SDS PAGE
GTX103252 diluted at 1:500

GTX103252_39918_IFA_19011506_588.jpg

GTX103252 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using COPD(GTX103252) antibody (green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (red) at 1:2500.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169