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COPS3 antibody

Anti-COPS3 antibody used in IHC (Paraffin sections) (IHC-P). GTX115102
Anti-COPS3 antibody used in Western Blot (WB). GTX115102
Anti-COPS3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115102

Cat. No. GTX115102

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all COPS3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.4 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human COPS3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11164093

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

COP9 signalosome subunit 3 , CSN3 , SGN3

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene possesses kinase activity that phosphorylates regulators involved in signal transduction. It phosphorylates I kappa-Balpha, p105, and c-Jun. It acts as a docking site for complex-mediated phosphorylation. The gene is located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-COPS3 antibody used in IHC (Paraffin sections) (IHC-P). GTX115102

GTX115102 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using COPS3(GTX115102) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-COPS3 antibody used in Western Blot (WB). GTX115102

GTX115102 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa
10% SDS PAGE
GTX115102 diluted at 1:3000

Anti-COPS3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115102

GTX115102 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using COPS3(GTX115102) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

REFERENCE

Application Reference

Xu GP et al. Biochem Biophys Res Commun 2013; 432 (3) : 425-30 Rig-G negatively regulates SCF-E3 ligase activities by disrupting the assembly of COP9 signalosome complex.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169