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COQ3 antibody

Anti-COQ3 antibody used in Western Blot (WB). GTX33110
Anti-COQ3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33110
Anti-COQ3 antibody used in IHC (Paraffin sections) (IHC-P). GTX33110

Cat No. GTX33110

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:50-1:200
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 50-369 of human COQ3 (NP_059117.3).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

coenzyme Q3, methyltransferase , DHHBMT , DHHBMTASE , UG0215E05 , bA9819.1

Cellular Localization

Mitochondrion inner membrane

Background

Ubiquinone, also known as coenzyme Q, or Q, is a critical component of the electron transport pathways of both eukaryotes and prokaryotes (Jonassen and Clarke, 2000 [PubMed 10777520]). This lipid consists of a hydrophobic isoprenoid tail and a quinone head group. The tail varies in length depending on the organism, but its purpose is to anchor coenzyme Q to the membrane. The quinone head group is responsible for the activity of coenzyme Q in the respiratory chain. The S. cerevisiae COQ3 gene encodes an O-methyltransferase required for 2 steps in the biosynthetic pathway of coenzyme Q. This enzyme methylates an early coenzyme Q intermediate, 3,4-dihydroxy-5-polyprenylbenzoic acid, as well as the final intermediate in the pathway, converting demethyl-ubiquinone to coenzyme Q. The COQ3 gene product is also capable of methylating the distinct prokaryotic early intermediate 2-hydroxy-6-polyprenyl phenol.[supplied by OMIM, Mar 2008]

Database

Research Area

DATA IMAGES

Anti-COQ3 antibody used in Western Blot (WB). GTX33110

GTX33110 WB Image

WB analysis of various sample lysates using GTX33110 COQ3 antibody.
Loading : 25ug per lane
Dilution : 1:1000

Anti-COQ3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33110

GTX33110 ICC/IF Image

ICC/IF analysis of A549 cells using GTX33110 COQ3 antibody.
Blue : DAPI

Anti-COQ3 antibody used in IHC (Paraffin sections) (IHC-P). GTX33110

GTX33110 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX33110 COQ3 antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf