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CORD2 antibody

Anti-CORD2 antibody used in IHC (Paraffin sections) (IHC-P). GTX124188
Anti-CORD2 antibody used in Western Blot (WB). GTX124188
Anti-CORD2 antibody used in Western Blot (WB). GTX124188
Anti-CORD2 antibody used in Western Blot (WB). GTX124188

Cat No. GTX124188

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

SK-N-SH , mouse eye

Predict Reactivity

Rat, Bovine, Cat, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CORD2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cone-rod homeobox , CORD2 , CRD , LCA7 , OTX3

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a photoreceptor-specific transcription factor which plays a role in the differentiation of photoreceptor cells. This homeodomain protein is necessary for the maintenance of normal cone and rod function. Mutations in this gene are associated with photoreceptor degeneration, Leber congenital amaurosis type III and the autosomal dominant cone-rod dystrophy 2. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CORD2 antibody used in IHC (Paraffin sections) (IHC-P). GTX124188

GTX124188 IHC-P Image

CORD2 antibody detects CORD2 protein expression by immunohistochemical analysis.
Sample: Paraffin-Embedded adult mouse retina.
Green: CORD2 protein stained by CORD2 antibody (GTX124188) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CORD2 antibody used in Western Blot (WB). GTX124188

GTX124188 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with CORD2 antibody (GTX124188) diluted at 1:1000.

Anti-CORD2 antibody used in Western Blot (WB). GTX124188

GTX124188 WB Image

Sample (30 ug of whole cell lysate)
A: SK-N-SH
12% SDS PAGE
GTX124188 diluted at 1:1000

Anti-CORD2 antibody used in Western Blot (WB). GTX124188

GTX124188 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with CORD2 antibody (GTX124188) diluted at 1:1000.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169