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COUP TF1 antibody [N1], N-term

Anti-COUP TF1 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104801
Anti-COUP TF1 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX104801
Anti-COUP TF1 antibody [N1], N-term used in Western Blot (WB). GTX104801

Cat No. GTX104801

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

COUP TF1 tansfected 293T cell

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human COUP TF1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241122

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

nuclear receptor subfamily 2 group F member 1 , BBOAS , BBSOAS , COUP-TFI , COUPTF1 , EAR-3 , EAR3 , ERBAL3 , SVP44 , TCFCOUP1 , TFCOUP1

Background

Coup (chicken ovalbumin upstream promoter) transcription factor binds to the ovalbumin promoter and, in conjunction with another protein (S300-II) stimulates initiation of transcription. Binds to both direct repeats and palindromes of the 5'-AGGTCA-3' motif. Represses transcriptional activity of LHCG.

Database

DATA IMAGES

Anti-COUP TF1 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104801

GTX104801 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using COUP TF1 (GTX104801) antibody at 1:200 dilution.

Anti-COUP TF1 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX104801

GTX104801 IHC-P Image

COUP TF1 antibody [N1], N-term detects COUP TF1 protein at nucleus on mouse hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hind brain.
COUP TF1 antibody [N1], N-term (GTX104801) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-COUP TF1 antibody [N1], N-term used in Western Blot (WB). GTX104801

GTX104801 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with COUP TF1 antibody [N1], N-term (GTX104801) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169