GeneTex
United States (US)

COUP TF1 antibody [N1], N-term

GTX104801_39708_20181109_WB_B_19011519_146.jpg
GTX104801_WB_19011506_564.jpg
GTX104801_IFA_19011506_997.jpg
GTX104801_39708_20141114_IHC_M_19011506_501.jpg

Cat No. GTX104801

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

COUP TF1 tansfected 293T cell

Predict Reactivity

Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human COUP TF1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nuclear Receptor Subfamily 2 Group F Member 1,Bboas,Bbsoas,Coup-Tfi,Couptf1,Ear-3,Ear3,Erbal3,Svp44,Tcfcoup1,Tfcoup1,Nr2F1

Background

Coup (chicken ovalbumin upstream promoter) transcription factor binds to the ovalbumin promoter and, in conjunction with another protein (S300-II) stimulates initiation of transcription. Binds to both direct repeats and palindromes of the 5'-AGGTCA-3' motif. Represses transcriptional activity of LHCG.

Database

DATA IMAGES
GTX104801_39708_20181109_WB_B_19011519_146.jpg

GTX104801 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with COUP TF1 antibody [N1], N-term (GTX104801) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104801_WB_19011506_564.jpg

GTX104801 WB Image

Western Blot analysis of NR2F1 expression in transfected 293T cell line by NR2F1 polyclonal antibody.
A: Non-transfected lysate.
B: NR2F1 transfected lysate.
12% SDS PAGE
GTX104801 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104801_IFA_19011506_997.jpg

GTX104801 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using COUP TF1 (GTX104801) antibody at 1:200 dilution.

GTX104801_39708_20141114_IHC_M_19011506_501.jpg

GTX104801 IHC-P Image

COUP TF1 antibody [N1], N-term detects COUP TF1 protein at nucleus on mouse hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hind brain.
COUP TF1 antibody [N1], N-term (GTX104801) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169