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COUP TF1 antibody [N1], N-term

Anti-COUP TF1 antibody [N1], N-term used in Western Blot (WB). GTX104801
Anti-COUP TF1 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX104801
Anti-COUP TF1 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104801

Cat. No. GTX104801

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all COUP TF1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

NR2F1-transfected 293T

Predict Reactivity

Rat, Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human COUP TF1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241122

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear receptor subfamily 2 group F member 1 , BBOAS , BBSOAS , COUP-TFI , COUPTF1 , EAR-3 , EAR3 , ERBAL3 , SVP44 , TCFCOUP1 , TFCOUP1

Background

Coup (chicken ovalbumin upstream promoter) transcription factor binds to the ovalbumin promoter and, in conjunction with another protein (S300-II) stimulates initiation of transcription. Binds to both direct repeats and palindromes of the 5'-AGGTCA-3' motif. Represses transcriptional activity of LHCG.

Database

Research Area

DATA IMAGES

Anti-COUP TF1 antibody [N1], N-term used in Western Blot (WB). GTX104801

GTX104801 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with COUP TF1 antibody [N1], N-term (GTX104801) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-COUP TF1 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX104801

GTX104801 IHC-P Image

COUP TF1 antibody [N1], N-term detects COUP TF1 protein at nucleus on mouse hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hind brain.
COUP TF1 antibody [N1], N-term (GTX104801) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-COUP TF1 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104801

GTX104801 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using COUP TF1 (GTX104801) antibody at 1:200 dilution.

REFERENCE

Application Reference

Malchenko S et al. Gene 2014; 534 (2) : 400-7 Onset of rosette formation during spontaneous neural differentiation of hESC and hiPSC colonies.
Application : ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169