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COUP TF1 antibody [N3C3]

Orthogonal Overexpression
Anti-COUP TF1 antibody [N3C3] used in Western Blot (WB). GTX114835
Anti-COUP TF1 antibody [N3C3] used in Western Blot (WB). GTX114835
Anti-COUP TF1 antibody [N3C3] used in Western Blot (WB). GTX114835

Cat. No. GTX114835

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all COUP TF1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IP Assay dependent
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

HCT116 , NR2F1-transfected 293T

Predict Reactivity

Rat, Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.92 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human COUP TF1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10724583

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear receptor subfamily 2 group F member 1 , BBOAS , BBSOAS , COUP-TFI , COUPTF1 , EAR-3 , EAR3 , ERBAL3 , SVP44 , TCFCOUP1 , TFCOUP1

Background

Coup (chicken ovalbumin upstream promoter) transcription factor binds to the ovalbumin promoter and, in conjunction with another protein (S300-II) stimulates initiation of transcription. Binds to both direct repeats and palindromes of the 5'-AGGTCA-3' motif. Represses transcriptional activity of LHCG.

Database

Research Area

DATA IMAGES

Anti-COUP TF1 antibody [N3C3] used in Western Blot (WB). GTX114835

GTX114835 WB Image

Sample (30 ug of whole cell lysate)
A: HCT116
10% SDS PAGE
GTX114835 diluted at 1:1000

Anti-COUP TF1 antibody [N3C3] used in Western Blot (WB). GTX114835

GTX114835 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with COUP TF1 antibody [N3C3] (GTX114835) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-COUP TF1 antibody [N3C3] used in Western Blot (WB). GTX114835

GTX114835 WB Image

Orthogonal

The data was published in the journal Nucleic Acids Res in 2013.PMID: 23666625

REFERENCE

Application Reference

Li Y et al. Nat Commun 2023; 14 (1) : 1732 A regulatory variant at 19p13.3 is associated with primary biliary cholangitis risk and ARID3A expression.
Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : IP
Reactivity : Human
Laursen KB et al. Nucleic Acids Res 2013; 41 (13) : 6430-43 Polycomb recruitment attenuates retinoic acid-induced transcription of the bivalent NR2F1 gene.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169