GeneTex
United States (US)

COUP TF1 antibody [N3C3]

Overexpression
GTX114835_40401_WB_18121410_998.jpg
GTX114835_40401_20181026_WB_B_19011519_272.jpg

Cat No. GTX114835

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IP Assay dependent

Note :

IP
target validated by Mass Spectrometry

Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

A549 , HeLa , HCT116

Predict Reactivity

Mouse, Rat, Bovine, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.92 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human COUP TF1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nuclear Receptor Subfamily 2 Group F Member 1,Bboas,Bbsoas,Coup-Tfi,Couptf1,Ear-3,Ear3,Erbal3,Svp44,Tcfcoup1,Tfcoup1,Nr2F1

Background

Coup (chicken ovalbumin upstream promoter) transcription factor binds to the ovalbumin promoter and, in conjunction with another protein (S300-II) stimulates initiation of transcription. Binds to both direct repeats and palindromes of the 5'-AGGTCA-3' motif. Represses transcriptional activity of LHCG.

Database

DATA IMAGES
GTX114835_40401_WB_18121410_998.jpg

GTX114835 WB Image

Sample (30 ug of whole cell lysate)
A: HCT116
10% SDS PAGE
GTX114835 diluted at 1:1000

GTX114835_40401_20181026_WB_B_19011519_272.jpg

GTX114835 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with COUP TF1 antibody [N3C3] (GTX114835) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169