GeneTex
  • Country / Location Selection

United States (US)

COUP TF1 antibody [N3C3]

Anti-COUP TF1 antibody [N3C3] used in Western Blot (WB). GTX114835
Anti-COUP TF1 antibody [N3C3] used in Western Blot (WB). GTX114835

Cat No. GTX114835

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IP Assay dependent
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

A549 , HeLa , HCT116

Predict Reactivity

Mouse, Rat, Bovine, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.92 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human COUP TF1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10724583

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

nuclear receptor subfamily 2 group F member 1 , BBOAS , BBSOAS , COUP-TFI , COUPTF1 , EAR-3 , EAR3 , ERBAL3 , SVP44 , TCFCOUP1 , TFCOUP1

Background

Coup (chicken ovalbumin upstream promoter) transcription factor binds to the ovalbumin promoter and, in conjunction with another protein (S300-II) stimulates initiation of transcription. Binds to both direct repeats and palindromes of the 5'-AGGTCA-3' motif. Represses transcriptional activity of LHCG.

Database

DATA IMAGES

Anti-COUP TF1 antibody [N3C3] used in Western Blot (WB). GTX114835

GTX114835 WB Image

Sample (30 ug of whole cell lysate)
A: HCT116
10% SDS PAGE
GTX114835 diluted at 1:1000

Anti-COUP TF1 antibody [N3C3] used in Western Blot (WB). GTX114835

GTX114835 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with COUP TF1 antibody [N3C3] (GTX114835) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

There are currently no reviews for COUP TF1 antibody [N3C3] (GTX114835). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169