GeneTex
United States (US)

COX1 antibody [C3], C-term

GTX104634_IHC_19011506_145.jpg
GTX104634_WB_19011506_371.jpg

Cat No. GTX104634

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Positive Control

293T , A431 , HepG2 , Molt-4 , Raji
PROPERTIES

Form

Liquid

Buffer

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human COX1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Prostaglandin-Endoperoxide Synthase 1,Cox1,Cox3,Pcox1,Pes-1,Pgg/Hs,Pghs-1,Pghs1,Phs1,Ptghs,Ptgs1

Cellular Localization

Microsome membrane; Peripheral membrane protein , Endoplasmic reticulum membrane; Peripheral membrane protein

Background

Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes PTGS1, which regulates angiogenesis in endothelial cells, and is inhibited by nonsteroidal anti-inflammatory drugs such as aspirin. PTGS1 is thought to be involved in cell-cell signaling and maintaining tissue homeostasis. Alternative splicing of this gene generates two transcript variants. The expression of these two transcripts is differentially regulated by relevant cytokines and growth factors. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX104634_IHC_19011506_145.jpg

GTX104634 IHC-P Image

Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using PTGS1 (GTX104634) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104634_WB_19011506_371.jpg

GTX104634 WB Image

Sample(30 μg of whole cell lysate)
A:MOLT4(GTX27912)
10% SDS PAGE
GTX104634 diluted at 1:500

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169