GeneTex
United States (US)

COX2 antibody, C-term

GTX15839_1190_WB_18121410_319.jpg
GTX15839_65_ICC-IF_18121410_983.jpg
GTX15839_30_FACS_18121410_259.jpg
GTX15839_66_ICC-IF_18121410_101.jpg

Cat No. GTX15839

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, FACS
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-0.3μg/ml
ICC/IF 10μg/ml
FACS Assay dependent

Note :

ICC/IF
Strong expression of the protein seen in the cytoplasm and vesicles of HepG2 and NIH3T3 cells.

Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX15839-PEP).

Predict Reactivity

Bovine, Dog, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-NPTVLLKERSTEL, from the C Terminus of the protein sequence according to NP_000954.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Prostaglandin-Endoperoxide Synthase 2,Cox-2,Cox2,Gripghs,Pgg/Hs,Pghs-2,Phs-2,Hcox-2,Ptgs2

Cellular Localization

Microsomal membrane

Background

Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. [provided by RefSeq, Feb 2009]

Database

Research Area

DATA IMAGES
GTX15839_1190_WB_18121410_319.jpg

GTX15839 WB Image

WB analysis of A549 (A) and Daudi (B) cell lysate using GTX15839 COX2 antibody, C-term.
Dilution : 0.1µg/ml
Loading : 35µg protein in RIPA buffer

GTX15839_65_ICC-IF_18121410_983.jpg

GTX15839 ICC/IF Image

ICC/IF analysis of PFA fixed HepG2 cells using GTX15839 COX2 antibody, C-term.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 10µg/ml

GTX15839_30_FACS_18121410_259.jpg

GTX15839 FACS Image

FACS analysis of PFA fixed HeLa cells using GTX15839 COX2 antibody, C-term.
Blue : Primary antibody
Black : Isotype control
Permeabilization : 0.5% Triton
Dilution : 10µg/ml

GTX15839_66_ICC-IF_18121410_101.jpg

GTX15839 ICC/IF Image

ICC/IF analysis of PFA fixed NIH-3T3 cells using GTX15839 COX2 antibody, C-term.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 10µg/ml

Package List Price ($)
$ 329