GeneTex
United States (US)

COX2 antibody [C3], C-term

Orthogonal
GTX100656_43159_20180316_WB_treatment_PMA_LPS_19011506_934.jpg
GTX100656_39988_20161208_WB_M_PMA_LPS_19011506_527.jpg
GTX100656_IHC_19011506_835.jpg

Cat No. GTX100656

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Positive Control

THP-1 (100 ng/ml PMA treatment for 16 hr and 1000 ng/ml LPS treatment for 24 hr) , RAW264.7 (0.1 μg/ml PMA treatment for 16 hr and 1 μg/ml LPS treatment for 24 hr)

Predict Reactivity

Sheep, Bovine, Cat, Dog, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.86mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human COX2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

COX-2 antibody, COX2 antibody, GRIPGHS antibody, PGG/HS antibody, PGHS-2 antibody, PHS-2 antibody, hCox-2 antibody, PTGS2 antibody, prostaglandin H2 synthase 2 antibody, cyclooxygenase 2b antibody, prostaglandin G/H synthase 2 antibody, PGH synthase 2 antibody, PHS II antibody, prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody

Background

Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100656_43159_20180316_WB_treatment_PMA_LPS_19011506_934.jpg

GTX100656 WB Image

Untreated (–) and treated (+) THP-1 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with COX2 antibody [C3], C-term (GTX100656) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100656_39988_20161208_WB_M_PMA_LPS_19011506_527.jpg

GTX100656 WB Image

Orthogonal

Untreated (–) and treated (+) RAW264.7 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with COX2 antibody [C3], C-term (GTX100656) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100656_IHC_19011506_835.jpg

GTX100656 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using COX2(GTX100656) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169