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COX2 antibody [C3], C-term

Anti-COX2 antibody [C3], C-term used in Western Blot (WB). GTX100656
Anti-COX2 antibody [C3], C-term used in Western Blot (WB). GTX100656
Anti-COX2 antibody [C3], C-term used in Western Blot (WB). GTX100656
Anti-COX2 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100656
Anti-COX2 antibody [C3], C-term used in Western Blot (WB). GTX100656

Cat. No. GTX100656

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Positive Control

RAW264.7 (0.1 μg/ml PMA treatment for 16 hr and 1 μg/ml LPS treatment for 24 hr) , THP-1 (100 ng/ml PMA treatment for 16 hr and 1000 ng/ml LPS treatment for 24 hr)

Predict Reactivity

Sheep, Bovine, Cat, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.42 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human COX2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951531

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

prostaglandin-endoperoxide synthase 2 , COX-2 , COX2 , GRIPGHS , PGG/HS , PGHS-2 , PHS-2 , hCox-2

Background

Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-COX2 antibody [C3], C-term used in Western Blot (WB). GTX100656

GTX100656 WB Image

Untreated (–) and treated (+) THP-1 whole cell extract (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with COX2 antibody [C3], C-term (GTX100656) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-COX2 antibody [C3], C-term used in Western Blot (WB). GTX100656

GTX100656 WB Image

Untreated (–) and treated (+) RAW264.7 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with COX2 antibody [C3], C-term (GTX100656) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-COX2 antibody [C3], C-term used in Western Blot (WB). GTX100656

GTX100656 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with COX2 antibody [C3], C-term (GTX100656) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Anti-COX2 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100656

GTX100656 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using COX2(GTX100656) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-COX2 antibody [C3], C-term used in Western Blot (WB). GTX100656

GTX100656 WB Image

The data was published in the journal Evid Based Complement Alternat Med in 2013. PMID: 23573126

REFERENCE

REVIEW

There are currently no reviews for COX2 antibody [C3], C-term (GTX100656). Be the first to share your experience with this product.
Application Reference
Chien LH et al. Antioxidants (Basel) 2021; 10 (10)
Application : WB
Reactivity : Mouse
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169