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COX4 antibody [6B3]

Anti-COX4 antibody [6B3] used in Western Blot (WB). GTX82782
Anti-COX4 antibody [6B3] used in Flow cytometry (FACS). GTX82782
Anti-COX4 antibody [6B3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82782

Cat. No. GTX82782

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 6B3

Isotype

 IgG1

Application

 WB, ICC/IF, FACS, ELISA

Reactivity

 Human, Mouse, Rat, Monkey
Package
100 μl ($399)
See all COX4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human COX4I1 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cytochrome c oxidase subunit 4I1 , COX IV-1 , COX4 , COX4-1 , COXIV , COXIV-1

Cellular Localization

Mitochondrion inner membrane

Background

Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-COX4 antibody [6B3] used in Western Blot (WB). GTX82782

GTX82782 WB Image

WB analysis of HEK293 (1), A549 (2) and PC12 (3) cell lysate using GTX82782 COX4 antibody [6B3].

Anti-COX4 antibody [6B3] used in Flow cytometry (FACS). GTX82782

GTX82782 FACS Image

FACS analysis of K562 cells using GTX82782 COX4 antibody [6B3].
Blue : COX4
Red : negative control

Anti-COX4 antibody [6B3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82782

GTX82782 ICC/IF Image

ICC/IF analysis of PANC-1 cells using GTX82782 COX4 antibody [6B3].
Green : COX4
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399