Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [GT6310] (GTX628886) diluted at 1:1500.

COX4 antibody [GT6310] detects COX4I1 protein by Western blot analysis.
A. 50 μg rat muscle lysate/extract
12 % SDS-PAGE
COX4 antibody [GT6310] (GTX628886) dilution: 1:1000

COX4 antibody [GT6310] detects COX4I1 protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
COX4 antibody [GT6310] (GTX628886) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

COX4 antibody [GT6310] detects COX4I1 protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
COX4 antibody [GT6310] (GTX628886) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

COX4I1 antibody detects COX4I1 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in -20ºC 100% methanol for 5 min.
Green: COX4I1 protein stained by COX4I1 antibody (GTX628886) diluted at 1:500.
Blue: Hoechst 33342 staining.

COX4 antibody [GT6310] detects COX4 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
COX4 stained by COX4 antibody [GT6310] (GTX628886) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [GT6310] (GTX628886) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.