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COX4 antibody [GT6310]

KO/KD
Anti-COX4 antibody [GT6310] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628886
Anti-COX4 antibody [GT6310] used in Western Blot (WB). GTX628886
Anti-COX4 antibody [GT6310] used in IHC (Paraffin sections) (IHC-P). GTX628886
Anti-COX4 antibody [GT6310] used in Western Blot (WB). GTX628886
Anti-COX4 antibody [GT6310] used in IHC (Paraffin sections) (IHC-P). GTX628886
Anti-COX4 antibody [GT6310] used in Western Blot (WB). GTX628886

Cat. No. GTX628886

Reference 4

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT6310

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all COX4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , A375 , rat muscle

Predict Reactivity

Bovine, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human COX4. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888063

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cytochrome c oxidase subunit 4I1 , COX IV-1 , COX4 , COX4-1 , COXIV , COXIV-1

Cellular Localization

Mitochondrion inner membrane

Background

Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-COX4 antibody [GT6310] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628886

GTX628886 ICC/IF Image

COX4I1 antibody detects COX4I1 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in -20ºC 100% methanol for 5 min.
Green: COX4I1 protein stained by COX4I1 antibody (GTX628886) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-COX4 antibody [GT6310] used in Western Blot (WB). GTX628886

GTX628886 WB Image

COX4 antibody [GT6310] detects COX4I1 protein by Western blot analysis.
A. 50 μg rat muscle lysate/extract
12 % SDS-PAGE
COX4 antibody [GT6310] (GTX628886) dilution: 1:1000

Anti-COX4 antibody [GT6310] used in IHC (Paraffin sections) (IHC-P). GTX628886

GTX628886 IHC-P Image

COX4 antibody [GT6310] detects COX4I1 protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
COX4 antibody [GT6310] (GTX628886) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-COX4 antibody [GT6310] used in Western Blot (WB). GTX628886

GTX628886 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [GT6310] (GTX628886) diluted at 1:1500.

Anti-COX4 antibody [GT6310] used in IHC (Paraffin sections) (IHC-P). GTX628886

GTX628886 IHC-P Image

COX4 antibody [GT6310] detects COX4I1 protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
COX4 antibody [GT6310] (GTX628886) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-COX4 antibody [GT6310] used in Western Blot (WB). GTX628886

GTX628886 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [GT6310] (GTX628886) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Naeem S et al. Toxicol Lett 2020; 326 : 1-10 NIX compensates lost role of parkin in cd-induced mitophagy in HeLa cells through phosphorylation.
Application : WB, ICC/IF
Reactivity : Human
Choi BW et al. Nucl Med Mol Imaging 2019; 53 (6) : 396-405 Reverse Warburg Effect-Related Mitochondrial Activity and 18F-FDG Uptake in Invasive Ductal Carcinoma.
Gu X et al. Toxicol Lett 2018; (Epub) Lead (Pb) induced ATM-dependent mitophagy via PINK1/Parkin pathway.
Application : WB, ICC/IF
Reactivity : Human
Jeong SA et al. Oncotarget 2015; 6 (25) : 21173-92 Ca2+ influx-mediated dilation of the endoplasmic reticulum and c-FLIPL downregulation trigger CDDO-Me-induced apoptosis in breast cancer cells.
Application : ICC/IF
Reactivity : Human
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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations