APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2 , A375 , rat muscle
Predict Reactivity
Bovine, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS pH7, 20% Glycerol
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human COX4. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888063
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
cytochrome c oxidase subunit 4I1 , COX IV-1 , COX4 , COX4-1 , COXIV , COXIV-1
Cellular Localization
Mitochondrion inner membrane
Background
Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX628886 ICC/IF Image
COX4I1 antibody detects COX4I1 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in -20ºC 100% methanol for 5 min. Green: COX4I1 protein stained by COX4I1 antibody (GTX628886) diluted at 1:500. Blue: Hoechst 33342 staining.
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GTX628886 WB Image
COX4 antibody [GT6310] detects COX4I1 protein by Western blot analysis. A. 50 μg rat muscle lysate/extract 12 % SDS-PAGE COX4 antibody [GT6310] (GTX628886) dilution: 1:1000
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GTX628886 IHC-P Image
COX4 antibody [GT6310] detects COX4I1 protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. COX4 antibody [GT6310] (GTX628886) dilution: 1:200.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX628886 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [GT6310] (GTX628886) diluted at 1:1500.
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GTX628886 IHC-P Image
COX4 antibody [GT6310] detects COX4I1 protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. COX4 antibody [GT6310] (GTX628886) dilution: 1:200.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX628886 WB Image
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [GT6310] (GTX628886) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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REFERENCE
REVIEW
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