GeneTex
United States (US)

COX4 antibody [GT6310]

KO/KD
GTX628886_41239_IFA_18121411_380.jpg
GTX628886_41239_WB_R_muscle_18121411_498.jpg
GTX628886_41239_WB_18121411_762.jpg
GTX628886_41239_IHC_2_18121411_118.jpg
GTX628886_41239_20160721_WB_shRNA_watermark_18121411_347.jpg
GTX628886_41239_IHC_18121411_427.jpg

Cat No. GTX628886

Reference 3
Host Mouse
Clonality Monoclonal
Clone Name GT6310
Isotype IgG2b
Application WB, ICC/IF, IHC-P
Reactivity Human, Rat
See all COX4 products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , A375 , rat muscle

Predict Reactivity

Bovine, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human COX4. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cytochrome C Oxidase Subunit 4I1,Cox Iv-1,Cox4,Cox4-1,Coxiv,Coxiv-1,Cox4I1

Cellular Localization

Mitochondrion inner membrane

Background

Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX628886_41239_IFA_18121411_380.jpg

GTX628886 ICC/IF Image

COX4I1 antibody detects COX4I1 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in -20℃ 100% methanol for 5 min.
Green: COX4I1 protein stained by COX4I1 antibody (GTX628886) diluted at 1:500.
Blue: Hoechst 33342 staining.
GTX628886_41239_WB_R_muscle_18121411_498.jpg

GTX628886 WB Image

COX4 antibody [GT6310] detects COX4I1 protein by Western blot analysis.
A. 50 µg rat muscle lysate/extract
12 % SDS-PAGE
COX4 antibody [GT6310] (GTX628886) dilution: 1:1000
GTX628886_41239_WB_18121411_762.jpg

GTX628886 WB Image

COX4 antibody [GT6310] detects COX4I1 protein by Western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg A431 whole cell lysate/extract
C. 30 µg HeLa whole cell lysate/extract
D. 30 µg HepG2 whole cell lysate/extract
E. 30 µg A375 whole cell lysate/extract
12 % SDS-PAGE
COX4 antibody [GT6310] (GTX628886) dilution: 1:1000
GTX628886_41239_IHC_2_18121411_118.jpg

GTX628886 IHC-P Image

COX4 antibody [GT6310] detects COX4I1 protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
COX4 antibody [GT6310] (GTX628886) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
GTX628886_41239_20160721_WB_shRNA_watermark_18121411_347.jpg

GTX628886 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [GT6310] (GTX628886) diluted at 1:1500.
GTX628886_41239_IHC_18121411_427.jpg

GTX628886 IHC-P Image

COX4 antibody [GT6310] detects COX4I1 protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
COX4 antibody [GT6310] (GTX628886) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
REFERENCE

Application Reference

Gu X et al. Toxicol Lett 2018; 291 : 92-100 Lead (Pb) induced ATM-dependent mitophagy via PINK1/Parkin pathway.
Application : WB
Reactivity : Human
Gu X et al. Toxicol Lett 2018; (Epub ahead of print) Lead (Pb) induced ATM-dependent mitophagy via PINK1/Parkin pathway.
Application : WB, ICC/IF
Reactivity : Human
Jeong SA et al. Oncotarget 2015; 6 (25) : 21173-92 Ca2+ influx-mediated dilation of the endoplasmic reticulum and c-FLIPL downregulation trigger CDDO-Me-induced apoptosis in breast cancer cells.
Application : ICC/IF
Reactivity : Human
Submit a Reference
SDS
PBS.pdf
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