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COX4 antibody [GT854]

KO/KD Orthogonal
Anti-COX4 antibody [GT854] used in Western Blot (WB). GTX628901
Anti-COX4 antibody [GT854] used in Western Blot (WB). GTX628901
Anti-COX4 antibody [GT854] used in IHC (Paraffin sections) (IHC-P). GTX628901
Anti-COX4 antibody [GT854] used in IHC (Paraffin sections) (IHC-P). GTX628901
Anti-COX4 antibody [GT854] used in Western Blot (WB). GTX628901
Anti-COX4 antibody [GT854] used in Western Blot (WB). GTX628901
Anti-COX4 antibody [GT854] used in Western Blot (WB). GTX628901
Anti-COX4 antibody [GT854] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628901

Cat. No. GTX628901

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT854

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all COX4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

mouse heart , rat muscle , 293T

Predict Reactivity

Bovine, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human COX4. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888077

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cytochrome c oxidase subunit 4I1 , COX IV-1 , COX4 , COX4-1 , COXIV , COXIV-1

Cellular Localization

Mitochondrion inner membrane

Background

Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-COX4 antibody [GT854] used in Western Blot (WB). GTX628901

GTX628901 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [GT854] (GTX628901) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-COX4 antibody [GT854] used in Western Blot (WB). GTX628901

GTX628901 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [GT854] (GTX628901) diluted at 1:1500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-COX4 antibody [GT854] used in IHC (Paraffin sections) (IHC-P). GTX628901

GTX628901 IHC-P Image

COX4 antibody [GT854] detects COX4 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
COX4 stained by COX4 antibody [GT854] (GTX628901) diluted at 1:200.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min

Anti-COX4 antibody [GT854] used in IHC (Paraffin sections) (IHC-P). GTX628901

GTX628901 IHC-P Image

COX4 antibody [GT854] detects COX4 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian cancer.
COX4 stained by COX4 antibody [GT854] (GTX628901) diluted at 1:200.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min

Anti-COX4 antibody [GT854] used in Western Blot (WB). GTX628901

GTX628901 WB Image

Orthogonal

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [GT854] (GTX628901) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-COX4 antibody [GT854] used in Western Blot (WB). GTX628901

GTX628901 WB Image

COX4 antibody detects COX4I1 protein by western blot analysis.
A. 50 μg mouse heart lysate/extract
12% SDS-PAGE
COX4 antibody (GTX628901) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-COX4 antibody [GT854] used in Western Blot (WB). GTX628901

GTX628901 WB Image

COX4 antibody [GT854] detects COX4I1 protein by western blot analysis.
A. 50 μg rat muscle lysate/extract
15% SDS-PAGE
COX4 antibody [GT854] (GTX628901) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-COX4 antibody [GT854] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628901

GTX628901 ICC/IF Image

COX4 antibody detects COX4 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: COX4 stained by COX4 antibody (GTX628901) diluted at 1:1000.

REFERENCE

Application Reference

Zhao J et al. Br J Cancer 2021; 124 (2) : 425-436 G3BP1 interacts with YWHAZ to regulate chemoresistance and predict adjuvant chemotherapy benefit in gastric cancer.
Peri? I et al. Neuroscience 2020; 451 : 111-125 Tianeptine Enhances Energy-related Processes in the Hippocampal Non-synaptic Mitochondria in a Rat Model of Depression.
Application : WB
Reactivity : Rat
Dongil P et al. Aging (Albany NY) 2020; 12 (3) : 2275-2301 PAS kinase deficiency reduces aging effects in mice.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations