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COX4 antibody [HL2264]

Recombinant antibody KO/KD
Anti-COX4 antibody [HL2264] used in Western Blot (WB). GTX638316
Anti-COX4 antibody [HL2264] used in Western Blot (WB). GTX638316
Anti-COX4 antibody [HL2264] used in IHC (Paraffin sections) (IHC-P). GTX638316
Anti-COX4 antibody [HL2264] used in Western Blot (WB). GTX638316
Anti-COX4 antibody [HL2264] used in Western Blot (WB). GTX638316
Anti-COX4 antibody [HL2264] used in Western Blot (WB). GTX638316
Anti-COX4 antibody [HL2264] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638316
Anti-COX4 antibody [HL2264] used in Western Blot (WB). GTX638316

Cat. No. GTX638316

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2264

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all COX4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human COX4.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cytochrome c oxidase subunit 4I1 , COX IV-1 , COX4 , COX4-1 , COXIV , COXIV-1

Cellular Localization

Mitochondrion inner membrane

Background

Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. Pseudogenes related to this gene are located on chromosomes 13 and 14. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016]

Database

Research Area

DATA IMAGES

Anti-COX4 antibody [HL2264] used in Western Blot (WB). GTX638316

GTX638316 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [HL2264] (GTX638316) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-COX4 antibody [HL2264] used in Western Blot (WB). GTX638316

GTX638316 WB Image

Whole zebrafish extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [HL2264] (GTX638316) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-COX4 antibody [HL2264] used in IHC (Paraffin sections) (IHC-P). GTX638316

GTX638316 IHC-P Image

COX4 antibody [HL2264] detects COX4 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
COX4 stained by COX4 antibody [HL2264] (GTX638316) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-COX4 antibody [HL2264] used in Western Blot (WB). GTX638316

GTX638316 WB Image

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [HL2264] (GTX638316) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-COX4 antibody [HL2264] used in Western Blot (WB). GTX638316

GTX638316 WB Image

Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [HL2264] (GTX638316) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-COX4 antibody [HL2264] used in Western Blot (WB). GTX638316

GTX638316 WB Image

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [HL2264] (GTX638316) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Anti-COX4 antibody [HL2264] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638316

GTX638316 ICC/IF Image

COX4 antibody [HL2264] detects COX4 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: COX4 stained by COX4 antibody [HL2264] (GTX638316) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-COX4 antibody [HL2264] used in Western Blot (WB). GTX638316

GTX638316 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [HL2264] (GTX638316) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations