GeneTex
United States (US)

COX4 antibody

KO/KD
GTX101499_39771_20160721_WB_shRNA_watermark_19011506_676.jpg
GTX101499_WB_19011506_996.jpg
GTX101499_39771_IHC_19011506_575.jpg
GTX101499_39771_IFA_19011506_544.jpg
GTX101499_39771_20150604_IP_19011506_929.jpg

Cat No. GTX101499

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji

Predict Reactivity

Bovine, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.38mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human COX4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

COX4 antibody, COX4-1 antibody, COXIV antibody, FLJ23483 antibody, MGC72016 antibody, COX4I1 antibody, "cytochrome c oxidase subunit 4 isoform 1, mitochondrial antibody", COX IV-1 antibody, cytochrome c oxidase polypeptide IV antibody, cytochrome c oxidase subunit IV isoform 1 antibody

Cellular Localization

Mitochondrion inner membrane

Background

Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101499_39771_20160721_WB_shRNA_watermark_19011506_676.jpg

GTX101499 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody (GTX101499) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101499_WB_19011506_996.jpg

GTX101499 WB Image

Sample(30 µg of whole cell lysate)
A:A431(GTX27909)
B:MOLT4(GTX27912)
12% SDS PAGE
GTX101499 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101499_39771_IHC_19011506_575.jpg

GTX101499 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CA922 xenograft, using COX4(GTX101499) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101499_39771_IFA_19011506_544.jpg

GTX101499 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using COX4(GTX101499) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

GTX101499_39771_20150604_IP_19011506_929.jpg

GTX101499 IP Image

Immunoprecipitation of COX4 protein from 293T whole cell extracts using 5 μg of COX4 antibody (GTX101499).
Western blot analysis was performed using COX4 antibody (GTX101499).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169