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COX4 antibody

KO/KD Orthogonal
Anti-COX4 antibody used in Western Blot (WB). GTX101499
Anti-COX4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101499
Anti-COX4 antibody used in IHC (Paraffin sections) (IHC-P). GTX101499
Anti-COX4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101499
Anti-COX4 antibody used in Immunoprecipitation (IP). GTX101499
Anti-COX4 antibody used in Western Blot (WB). GTX101499
Anti-COX4 antibody used in Western Blot (WB). GTX101499
Anti-COX4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101499
Anti-COX4 antibody used in Western Blot (WB). GTX101499

Cat No. GTX101499

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human
Package
100 μl ($329),
25 μl ($169)
See all COX4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji

Predict Reactivity

Bovine, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.38 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human COX4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cytochrome c oxidase subunit 4I1 , COX IV-1 , COX4 , COX4-1 , COXIV , COXIV-1

Cellular Localization

Mitochondrion inner membrane

Background

Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-COX4 antibody used in Western Blot (WB). GTX101499

GTX101499 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody (GTX101499) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-COX4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101499

GTX101499 ICC/IF Image

The ICC/IF analysis of COX4 antibody was published by Qi Y and colleagues in the journal Sci Rep in 2016.PMID: 27089984

Anti-COX4 antibody used in IHC (Paraffin sections) (IHC-P). GTX101499

GTX101499 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CA922 xenograft, using COX4(GTX101499) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-COX4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101499

GTX101499 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using COX4(GTX101499) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-COX4 antibody used in Immunoprecipitation (IP). GTX101499

GTX101499 IP Image

Immunoprecipitation of COX4 protein from 293T whole cell extracts using 5 μg of COX4 antibody (GTX101499).
Western blot analysis was performed using COX4 antibody (GTX101499).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-COX4 antibody used in Western Blot (WB). GTX101499

GTX101499 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody (GTX101499) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-COX4 antibody used in Western Blot (WB). GTX101499

GTX101499 WB Image

Orthogonal

The WB analysis of COX4 antibody was published by Qi Y and colleagues in the journal Sci Rep in 2016.PMID: 27089984

Anti-COX4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101499

GTX101499 ICC/IF Image

The ICC/IF analysis of COX4 antibody was published by Qi Y and colleagues in the journal Sci Rep in 2016.PMID: 27089984

Anti-COX4 antibody used in Western Blot (WB). GTX101499

GTX101499 WB Image

The WB analysis of COX4 antibody was published by Li CJ and colleagues in the journal Sci Rep in 2015.PMID: 25909282

REFERENCE

Application Reference

Qi Y et al. Sci Rep 2016; 6 : 24700 ATM mediates spermidine-induced mitophagy via PINK1 and Parkin regulation in human fibroblasts.
Application : WB, ICC/IF
Reactivity : Human
Li CJ et al. Sci Rep 2015; (Epub) Synergistic protection of N-acetylcysteine and ascorbic acid 2-phosphate on human mesenchymal stem cells against mitoptosis, necroptosis and apoptosis.
Application : WB
Reactivity : Human
Er-Chieh Cho et al. Mediators of Inflammation 2015; RRM2B-Mediated Regulation of Mitochondrial Activity and Inflammation under Oxidative Stress
Application : WB
Reactivity : Human
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169