*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji
Bovine, Chimpanzee, Rhesus Monkey(>80% identity)
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.38mg/ml(Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human COX4. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
COX4 antibody, COX4-1 antibody, COXIV antibody, FLJ23483 antibody, MGC72016 antibody, COX4I1 antibody, "cytochrome c oxidase subunit 4 isoform 1, mitochondrial antibody", COX IV-1 antibody, cytochrome c oxidase polypeptide IV antibody, cytochrome c oxidase subunit IV isoform 1 antibody
Mitochondrion inner membrane
Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. [provided by RefSeq]
GTX101499 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody (GTX101499) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX101499 WB Image
Sample(30 µg of whole cell lysate)
12% SDS PAGE
GTX101499 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX101499 IHC-P Image
Immunohistochemical analysis of paraffin-embedded CA922 xenograft, using COX4(GTX101499) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
GTX101499 ICC/IF Image
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using COX4(GTX101499) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
GTX101499 IP Image
Immunoprecipitation of COX4 protein from 293T whole cell extracts using 5 μg of COX4 antibody (GTX101499).
Western blot analysis was performed using COX4 antibody (GTX101499).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.