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COX4 antibody

KO/KD
Anti-COX4 antibody used in IHC (Paraffin sections) (IHC-P). GTX101499
Anti-COX4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101499
Anti-COX4 antibody used in Immunoprecipitation (IP). GTX101499
Anti-COX4 antibody used in Western Blot (WB). GTX101499
Anti-COX4 antibody used in Western Blot (WB). GTX101499

Cat No. GTX101499

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human
Package
100 μl ($329),
25 μl ($169)
See all COX4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji

Predict Reactivity

Bovine, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.38 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human COX4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cytochrome c oxidase subunit 4I1 , COX IV-1 , COX4 , COX4-1 , COXIV , COXIV-1

Cellular Localization

Mitochondrion inner membrane

Background

Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-COX4 antibody used in IHC (Paraffin sections) (IHC-P). GTX101499

GTX101499 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CA922 xenograft, using COX4(GTX101499) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-COX4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101499

GTX101499 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using COX4(GTX101499) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-COX4 antibody used in Immunoprecipitation (IP). GTX101499

GTX101499 IP Image

Immunoprecipitation of COX4 protein from 293T whole cell extracts using 5 μg of COX4 antibody (GTX101499).
Western blot analysis was performed using COX4 antibody (GTX101499).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-COX4 antibody used in Western Blot (WB). GTX101499

GTX101499 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody (GTX101499) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-COX4 antibody used in Western Blot (WB). GTX101499

GTX101499 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody (GTX101499) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Qi Y et al. Sci Rep 2016; 6 : 24700 ATM mediates spermidine-induced mitophagy via PINK1 and Parkin regulation in human fibroblasts.
Application : WB, ICC/IF
Reactivity : Human
Li CJ et al. Sci Rep 2015; (Epub) Synergistic protection of N-acetylcysteine and ascorbic acid 2-phosphate on human mesenchymal stem cells against mitoptosis, necroptosis and apoptosis.
Application : WB
Reactivity : Human
Er-Chieh Cho et al. Mediators of Inflammation 2015; RRM2B-Mediated Regulation of Mitochondrial Activity and Inflammation under Oxidative Stress
Application : WB
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169