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COX4 antibody

Anti-COX4 antibody used in Western Blot (WB). GTX114330
Anti-COX4 antibody used in Western Blot (WB). GTX114330
Anti-COX4 antibody used in Western Blot (WB). GTX114330
Anti-COX4 antibody used in IHC (Paraffin sections) (IHC-P). GTX114330
Anti-COX4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114330
Anti-COX4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114330
Anti-COX4 antibody used in Western Blot (WB). GTX114330
Anti-COX4 antibody used in Western Blot (WB). GTX114330

Cat. No. GTX114330

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

mouse muscle , rat muscle , 293T

Predict Reactivity

Rabbit, Bovine, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.42 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human COX4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10625002

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cytochrome c oxidase subunit 4I1 , COX IV-1 , COX4 , COX4-1 , COXIV , COXIV-1

Cellular Localization

Mitochondrion inner membrane

Background

Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-COX4 antibody used in Western Blot (WB). GTX114330

GTX114330 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody (GTX114330) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-COX4 antibody used in Western Blot (WB). GTX114330

GTX114330 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody (GTX114330) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-COX4 antibody used in Western Blot (WB). GTX114330

GTX114330 WB Image

Sample (50 μg of whole cell lysate)
A: rat muscle
12% SDS PAGE
GTX114330 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-COX4 antibody used in IHC (Paraffin sections) (IHC-P). GTX114330

GTX114330 IHC-P Image

COX4 antibody detects COX4 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
COX4 stained by COX4 antibody (GTX114330) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-COX4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114330

GTX114330 ICC/IF Image

COX4 antibody detects COX4 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: COX4 stained by COX4 antibody (GTX114330) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Anti-COX4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114330

GTX114330 ICC/IF Image

COX4 antibody detects COX4 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: COX4 stained by COX4 antibody (GTX114330) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Anti-COX4 antibody used in Western Blot (WB). GTX114330

GTX114330 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody (GTX114330) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-COX4 antibody used in Western Blot (WB). GTX114330

GTX114330 WB Image

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody (GTX114330) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

COX4 antibody Cat. No. GTX114330

Rating ( Average 4.9 based on 7 users reviews)
Application
Western Blot(WB) ( Average 5 based on 2 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 4 based on 1 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 4 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oxid Med Cell Longev in 2019.PMID: 31249652
Date : Anonymous submitted on 12-May-2021
Score :
Application Tested : WB
Sample Species : Ms
Sample : Synapotosome isolated from mouse brain tissue
Amount used : 10-20 μg
Blocking : 3% milk powder in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 8Hr
Figure Note : Numbers indicated separate mice; L: left and R: right hippocampus and prefrontal cortex (PFC).
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2017. PMID: 29233981
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2017. PMID: 29233981
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2017. PMID: 29233981
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2017. PMID: 29233981
Date : Anonymous submitted on 09-Dec-2013
Score :
Application Tested : ICC/IF
Sample Species : Ms
Sample : striatal progenitor cells
Blocking : 2% BSA, 2% normal goat serum in PBS, 25°C, 1.5Hr
Fixation : 4% paraformaldehyde, 4% sucrose in PBS
Permeabilization /Antigen retrieval : 0.05% NP40/PBS
Primary Antibody : 1:250, 4°C, 18Hr
Figure Note : GTX114330(Red),Hoechst 33258(Blue)
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169