GeneTex
United States (US)

COX4 antibody

KO/KD
GTX114330_41794_20160721_WB_shRNA_watermark_18121410_119.jpg
GTX114330_40184_IFA_18121410_800.jpg
GTX114330_40184_IHC_18121410_968.jpg
GTX114330_40184_WB_M_Muscle_18121410_302.jpg
GTX114330_40184_WB_R_Muscle_18121410_842.jpg
GTX114330_42550_20160728_WB_18121410_626.jpg
GTX114330_42550_20160817_IFA_18121410_955.jpg

Cat No. GTX114330

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2, mouse muscle , rat muscle , *mouse striatal progenitor cells

Predict Reactivity

Bovine, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.23mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human COX4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

COX4 antibody, COX4-1 antibody, COXIV antibody, FLJ23483 antibody, MGC72016 antibody, COX4I1 antibody, "cytochrome c oxidase subunit 4 isoform 1, mitochondrial antibody", COX IV-1 antibody, cytochrome c oxidase polypeptide IV antibody, cytochrome c oxidase subunit IV isoform 1 antibody

Cellular Localization

Mitochondrion inner membrane

Background

Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX114330_41794_20160721_WB_shRNA_watermark_18121410_119.jpg

GTX114330 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody (GTX114330) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX114330_40184_IFA_18121410_800.jpg

GTX114330 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using COX4(GTX114330) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

GTX114330_40184_IHC_18121410_968.jpg

GTX114330 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HS578T xenograft, using COX4(GTX114330) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX114330_40184_WB_M_Muscle_18121410_302.jpg

GTX114330 WB Image

Sample (50 µg of whole cell lysate)
A: mouse muscle
12% SDS PAGE
GTX114330 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX114330_40184_WB_R_Muscle_18121410_842.jpg

GTX114330 WB Image

Sample (50 µg of whole cell lysate)
A: rat muscle
12% SDS PAGE
GTX114330 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX114330_42550_20160728_WB_18121410_626.jpg

GTX114330 WB Image

Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody (GTX114330) diluted at 1:1000.

GTX114330_42550_20160817_IFA_18121410_955.jpg

GTX114330 ICC/IF Image

COX4 antibody detects COX4 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: COX4 protein stained by COX4 antibody (GTX114330) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE
REVIEW

COX4 antibody Cat No. GTX114330

Rating ( Average 4 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 09-Dec-2013
Score :
Application Tested : ICC/IF
Sample Species : Ms
Sample : striatal progenitor cells
Blocking : 2% BSA, 2% normal goat serum in PBS, 25°C, 1.5Hr
Fixation : 4% paraformaldehyde, 4% sucrose in PBS
Permeabilization /Antigen retrieval : 0.05% NP40/PBS
Primary Antibody : 1:250, 4°C, 18Hr
Figure Note : GTX114330(Red),Hoechst 33258(Blue)
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169