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COX4 antibody

KO/KD Orthogonal
Anti-COX4 antibody used in Western Blot (WB). GTX114330
Anti-COX4 antibody used in Western Blot (WB). GTX114330
Anti-COX4 antibody used in Western Blot (WB). GTX114330
Anti-COX4 antibody used in IHC (Paraffin sections) (IHC-P). GTX114330
Anti-COX4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114330
Anti-COX4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114330
Anti-COX4 antibody used in Western Blot (WB). GTX114330
Anti-COX4 antibody used in Western Blot (WB). GTX114330

Cat. No. GTX114330

Reference 27
7

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all COX4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

mouse muscle , rat muscle , 293T

Predict Reactivity

Rabbit, Bovine, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.42 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human COX4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10625002

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cytochrome c oxidase subunit 4I1 , COX IV-1 , COX4 , COX4-1 , COXIV , COXIV-1

Cellular Localization

Mitochondrion inner membrane

Background

Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-COX4 antibody used in Western Blot (WB). GTX114330

GTX114330 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody (GTX114330) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-COX4 antibody used in Western Blot (WB). GTX114330

GTX114330 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody (GTX114330) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-COX4 antibody used in Western Blot (WB). GTX114330

GTX114330 WB Image

Sample (50 μg of whole cell lysate)
A: rat muscle
12% SDS PAGE
GTX114330 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-COX4 antibody used in IHC (Paraffin sections) (IHC-P). GTX114330

GTX114330 IHC-P Image

COX4 antibody detects COX4 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
COX4 stained by COX4 antibody (GTX114330) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-COX4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114330

GTX114330 ICC/IF Image

COX4 antibody detects COX4 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: COX4 stained by COX4 antibody (GTX114330) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Anti-COX4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114330

GTX114330 ICC/IF Image

COX4 antibody detects COX4 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: COX4 stained by COX4 antibody (GTX114330) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Anti-COX4 antibody used in Western Blot (WB). GTX114330

GTX114330 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody (GTX114330) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-COX4 antibody used in Western Blot (WB). GTX114330

GTX114330 WB Image

Orthogonal

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody (GTX114330) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Zhewen Zhang et al. Curr Issues Mol Biol 2022; 44 (6) : 2759-2771 Parkin, as a Regulator, Participates in Arsenic Trioxide-Triggered Mitophagy in HeLa Cells.
Application : WB
Reactivity : Human
Chen CY et al. Sci Rep 2021; 11 (1) : 2377 Cardiac-specific microRNA-125b deficiency induces perinatal death and cardiac hypertrophy.
Application : WB
Reactivity : Mouse
Tsukahara T et al. Cell Signal 2021; 82 : 109951 Adenine nucleotide translocase 2, a putative target protein for 2-carba cyclic phosphatidic acid in microglial cells.
Szu-Yi Chou et al. Front Cell Dev Biol 2020; 8 : 564641 Altered Insulin Receptor Substrate 1 Phosphorylation in Blood Neuron-Derived Extracellular Vesicles From Patients With Parkinson's Disease.
Application : WB
Reactivity : Human
Lee H et al. J Biol Chem 2020; 295 (19) : 6543-6560 The short variant of optic atrophy 1 (OPA1) improves cell survival under oxidative stress.
Show More +
Submit a Reference

REVIEW

COX4 antibody Cat. No. GTX114330

Rating
( Average 4.9 based on 7 users reviews)
Application
Western Blot(WB)
( Average 5 based on 2 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 4 based on 1 users reviews)
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 4 users reviews)
Anti-COX4 antibody used in Western Blot (WB). GTX114330
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oxid Med Cell Longev in 2019.PMID: 31249652
Anti-COX4 antibody used in Western Blot (WB). GTX114330
Date : Anonymous submitted on 12-May-2021
Score :
Application Tested : WB
Sample Species : Ms
Sample : Synapotosome isolated from mouse brain tissue
Amount used : 10-20 μg
Blocking : 3% milk powder in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 8Hr
Figure Note : Numbers indicated separate mice; L: left and R: right hippocampus and prefrontal cortex (PFC).
Anti-COX4 antibody used in IHC (Paraffin sections) (IHC-P). GTX114330
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2017. PMID: 29233981
Anti-COX4 antibody used in IHC (Paraffin sections) (IHC-P). GTX114330
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2017. PMID: 29233981
Anti-COX4 antibody used in IHC (Paraffin sections) (IHC-P). GTX114330
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2017. PMID: 29233981
Anti-COX4 antibody used in IHC (Paraffin sections) (IHC-P). GTX114330
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Nat Commun in 2017. PMID: 29233981
Anti-COX4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114330
Date : Anonymous submitted on 09-Dec-2013
Score :
Application Tested : ICC/IF
Sample Species : Ms
Sample : striatal progenitor cells
Blocking : 2% BSA, 2% normal goat serum in PBS, 25°C, 1.5Hr
Fixation : 4% paraformaldehyde, 4% sucrose in PBS
Permeabilization /Antigen retrieval : 0.05% NP40/PBS
Primary Antibody : 1:250, 4°C, 18Hr
Figure Note : GTX114330(Red),Hoechst 33258(Blue)
Show More +
Submit a Review
Application Reference
Zhewen Zhang et al. Curr Issues Mol Biol 2022; 44 (6):2759-2771 Parkin, as a Regulator, Participates in Arsenic Trioxide-Triggered Mitophagy in HeLa Cells.
Application : WB
Reactivity : Human
Chen CY et al. Sci Rep 2021; 11 (1):2377 Cardiac-specific microRNA-125b deficiency induces perinatal death and cardiac hypertrophy.
Application : WB
Reactivity : Mouse
Tsukahara T et al. Cell Signal 2021; 82:109951 Adenine nucleotide translocase 2, a putative target protein for 2-carba cyclic phosphatidic acid in microglial cells.
Szu-Yi Chou et al. Front Cell Dev Biol 2020; 8:564641 Altered Insulin Receptor Substrate 1 Phosphorylation in Blood Neuron-Derived Extracellular Vesicles From Patients With Parkinson's Disease.
Application : WB
Reactivity : Human
Lee H et al. J Biol Chem 2020; 295 (19):6543-6560 The short variant of optic atrophy 1 (OPA1) improves cell survival under oxidative stress.
Mohd Salman et al. Dis Model Mech. 2020; 13 (8):dmm045021 Nrf2/HO-1 mediates the neuroprotective effects of pramipexole by attenuating oxidative damage and mitochondrial perturbation after traumatic brain injury in rats.
Application : WB
Reactivity : Rat
Tan C et al. J Cell Mol Med 2020; 24 (12):6833-6845 Seizure-induced impairment in neuronal ketogenesis: Role of zinc-α2-glycoprotein in mitochondria.
Application : WB
Reactivity : Rat
Shen M et al. Biomed Pharmacother 2020; 124:109911 Total glucosides of paeony decreases apoptosis of hepatocytes and inhibits maturation of dendritic cells in autoimmune hepatitis.
Application : WB
Reactivity : Mouse
Lu CC et al. Int J Oncol 2019; 54 (4):1271-1281 Metformin triggers the intrinsic apoptotic response in human AGS gastric adenocarcinoma cells by activating AMPK and suppressing mTOR/AKT signaling.
Lin TK et al. Oxid Med Cell Longev 2019; 2019:9537504 Mitochondrial Transfer of Wharton's Jelly Mesenchymal Stem Cells Eliminates Mutation Burden and Rescues Mitochondrial Bioenergetics in Rotenone-Stressed MELAS Fibroblasts.
Reactivity : Human
XiaotianZhang et al. Nutrition, Metabolism and Cardiovascular Diseases 2019; (Epub) MicroRNA-217-5p ameliorates endothelial cell apoptosis induced by ox-LDL by targeting CLIC4.
Gowri Nayak et al. bioRxiv 2019; (Epub) Adaptive thermogenesis in mice requires adipocyte light-sensing via Opsin 3.
Application : IHC-Fr
Reactivity : Mouse
Wang Y et al. Acta Histochem 2019; 121 (1):56-63 Protective effects of astaxanthin on subarachnoid hemorrhage-induced early brain injury: Reduction of cerebral vasospasm and improvement of neuron survival and mitochondrial function.
Application : WB
Reactivity : Rat
Tu HJ et al. Clin Epigenetics 2018; 10 (1):162 The anticancer effects of MPT0G211, a novel HDAC6 inhibitor, combined with chemotherapeutic agents in human acute leukemia cells.
Application : WB
Reactivity : Human
Fischer C et al. Nat Commun 2017; 8 (1):2079 A miR-327-FGF10-FGFR2-mediated autocrine signaling mechanism controls white fat browning.
Application : IHC-P
Reactivity : Mouse
Muller TA et al. Biochem Biophys Res Commun 2017; (Epub) Characterization of human AlkB homolog 1 produced in mammalian cells and demonstration of mitochondrial dysfunction in ALKBH1-deficient cells.
Application : WB
Reactivity : Human
Kuo SM et al. MBio 2017; 8 (3) Inhibition of Avian Influenza A Virus Replication in Human Cells by Host Restriction Factor TUFM Is Correlated with Autophagy.
Application : WB
Reactivity : Human
Lee H et al. J Biol Chem 2017; (Epub) The Short Variant of the Mitochondrial Dynamin OPA1 Maintains Mitochondrial Energetics and Cristae Structure.
Application : WB
Reactivity : Human
Chen YH et al. Journal of Diabetes Research 2016; 2016:Article ID 1828071 the different effects of * and * on cell death and parp activity in pancreatic nit-1 cells.
Application : WB
Reactivity : Mouse
Xu TJ et al. Eur Rev Med Pharmacol Sci 2016; 20 (15):3297-303 * in combination with selenium inhibits hg/pal-induced cardiomyocyte apoptosis by cbl-b regulating p38mapk/cbp/ku70 pathway.
Application : WB
Reactivity : Rat
Shindo Y et al. Sci Rep 2016; 6:29224 An Artificial Reaction Promoter Modulates Mitochondrial Functions via Chemically Promoting Protein Acetylation.
Application : WB
Reactivity : Human
Niu Z et al. Environ Toxicol Pharmacol 2016; 45:212-221 mitophagy inhibits proliferation by decreasing cyclooxygenase-2 (cox-2) in *-treated hepg2 cells.
Application : WB
Reactivity : Human
Cho EC et al. Mediators Inflamm 2015; 2015:287345 RRM2B-Mediated Regulation of Mitochondrial Activity and Inflammation under Oxidative Stress.
Application : WB
Reactivity : Human
Ishii Y et al. PLoS One 2015; 10 (10):e0140585 Knockout Serum Replacement Promotes Cell Survival by Preventing BIM from Inducing Mitochondrial Cytochrome C Release.
Application : WB
Reactivity : Human
Yi-Wen Lo et al. Journal of Cellular and Molecular Medicine 2015; 19 (4):744-59 mitochondrial proteomics with sirna knockdown to reveal acat1 and mdh2 in the development of *-resistant uterine cancer
Application : WB
Reactivity : Human
Cho EC et al. Oncotarget 2014; 5 (13):4834-44 Tumor suppressor FOXO3 regulates ribonucleotide reductase subunit RRM2B and impacts on survival of cancer patients.
Application : WB
Reactivity : Human
Hanaoka Y et al. PLoS One 2014; 9 (4):e94930 Tissue Inhibitor of Metalloproteinase-3 Knockout Mice Exhibit Enhanced Energy Expenditure through Thermogenesis.
Application : WB
Reactivity : Mouse
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169