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COX4 antibody

Anti-COX4 antibody used in Western Blot (WB). GTX114330
Anti-COX4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114330
Anti-COX4 antibody used in IHC (Paraffin sections) (IHC-P). GTX114330
Anti-COX4 antibody used in Western Blot (WB). GTX114330
Anti-COX4 antibody used in Western Blot (WB). GTX114330
Anti-COX4 antibody used in Western Blot (WB). GTX114330
Anti-COX4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114330

Cat No. GTX114330

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
Package
100μl ($319),
25μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2, mouse muscle , rat muscle , *mouse striatal progenitor cells

Predict Reactivity

Rabbit, Bovine, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.42 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human COX4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cytochrome C Oxidase Subunit 4I1,Cox Iv-1,Cox4,Cox4-1,Coxiv,Coxiv-1,Cox4I1

Cellular Localization

Mitochondrion inner membrane

Background

Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-COX4 antibody used in Western Blot (WB). GTX114330

GTX114330 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody (GTX114330) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-COX4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114330

GTX114330 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using COX4(GTX114330) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-COX4 antibody used in IHC (Paraffin sections) (IHC-P). GTX114330

GTX114330 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HS578T xenograft, using COX4(GTX114330) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-COX4 antibody used in Western Blot (WB). GTX114330

GTX114330 WB Image

Sample (50 µg of whole cell lysate)
A: mouse muscle
12% SDS PAGE
GTX114330 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-COX4 antibody used in Western Blot (WB). GTX114330

GTX114330 WB Image

Sample (50 µg of whole cell lysate)
A: rat muscle
12% SDS PAGE
GTX114330 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-COX4 antibody used in Western Blot (WB). GTX114330

GTX114330 WB Image

Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody (GTX114330) diluted at 1:1000.

Anti-COX4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114330

GTX114330 ICC/IF Image

COX4 antibody detects COX4 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: COX4 protein stained by COX4 antibody (GTX114330) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE
REVIEW

COX4 antibody Cat No. GTX114330

Rating ( Average 4 based on 1 users reviews)
Application
Date : Anonymous submitted on 09-Dec-2013
Score :
Application Tested : ICC/IF
Sample Species : Ms
Sample : striatal progenitor cells
Blocking : 2% BSA, 2% normal goat serum in PBS, 25°C, 1.5Hr
Fixation : 4% paraformaldehyde, 4% sucrose in PBS
Permeabilization /Antigen retrieval : 0.05% NP40/PBS
Primary Antibody : 1:250, 4°C, 18Hr
Figure Note : GTX114330(Red),Hoechst 33258(Blue)
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169