GeneTex

  • Country / Location Selection

United States (US)

COX5A antibody [N1C3]

Anti-COX5A antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101487
Anti-COX5A antibody [N1C3] used in Western Blot (WB). GTX101487
Anti-COX5A antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101487
Anti-COX5A antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101487
Anti-COX5A antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101487
Anti-COX5A antibody [N1C3] used in Western Blot (WB). GTX101487
Anti-COX5A antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101487
Anti-COX5A antibody [N1C3] used in Western Blot (WB). GTX101487

Cat. No. GTX101487

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all COX5A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Predict Reactivity

Rat, Bovine, Dog, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human COX5A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950021

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cytochrome c oxidase subunit 5A , COX , COX-VA , VA

Cellular Localization

Mitochondrion inner membrane

Background

Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer of proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Va of the human mitochondrial respiratory chain enzyme. A pseudogene COX5AP1 has been found in chromosome 14q22. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-COX5A antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101487

GTX101487 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using COX5A antibody [N1C3] (GTX101487) at 1:300 dilution.

Anti-COX5A antibody [N1C3] used in Western Blot (WB). GTX101487

GTX101487 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX5A antibody [N1C3] (GTX101487) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-COX5A antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101487

GTX101487 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using COX5A(GTX101487) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-COX5A antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101487

GTX101487 IHC-P Image

COX5A antibody [N1C3] detects COX5A protein at mitochondria on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
COX5A antibody [N1C3] (GTX101487) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-COX5A antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101487

GTX101487 IHC-P Image

COX5A antibody [N1C3] detects COX5A protein at mitochondria on mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
COX5A antibody [N1C3] (GTX101487) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-COX5A antibody [N1C3] used in Western Blot (WB). GTX101487

GTX101487 WB Image

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX5A antibody [N1C3] (GTX101487) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-COX5A antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101487

GTX101487 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using COX5A(GTX101487) antibody at 1:200 dilution.

Anti-COX5A antibody [N1C3] used in Western Blot (WB). GTX101487

GTX101487 WB Image

Sample (30 μg of whole cell lysate)
A: zebrafish eye
15% SDS PAGE
GTX101487 diluted at 1:1000

REFERENCE

There are currently no references for COX5A antibody [N1C3] (GTX101487). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for COX5A antibody [N1C3] (GTX101487). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169