Immunofluorescence analysis of methanol-fixed A431, using COX5A(GTX101487) antibody at 1:200 dilution.

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX5A antibody [N1C3] (GTX101487) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

COX5A antibody [N1C3] detects COX5A protein at mitochondria on mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
COX5A antibody [N1C3] (GTX101487) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

COX5A antibody [N1C3] detects COX5A protein at mitochondria on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
COX5A antibody [N1C3] (GTX101487) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using COX5A(GTX101487) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX5A antibody [N1C3] (GTX101487) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Sample (30 μg of whole cell lysate)
A: zebrafish eye
15% SDS PAGE
GTX101487 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using COX5A antibody [N1C3] (GTX101487) at 1:300 dilution.