GeneTex
United States (US)

COX5A antibody [N1C3]

GTX101487_39995_IHC_19011506_688.jpg
GTX101487_WB_19011506_922.jpg
GTX101487_39995_WB_M_brain_19011506_143.jpg
GTX101487_39995_IFA_19011506_414.jpg
GTX101487_39995_20150410_IHC_M_2_19011506_917.jpg
GTX101487_39995_20150410_IHC_M_19011506_593.jpg

Cat No. GTX101487

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse brain

Predict Reactivity

Rat, Bovine, Dog, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human COX5A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cytochrome C Oxidase Subunit 5A,Cox,Cox-Va,Va,Cox5A

Cellular Localization

Mitochondrion inner membrane

Background

Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer of proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Va of the human mitochondrial respiratory chain enzyme. A pseudogene COX5AP1 has been found in chromosome 14q22. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101487_39995_IHC_19011506_688.jpg

GTX101487 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using COX5A(GTX101487) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101487_WB_19011506_922.jpg

GTX101487 WB Image

Sample(30 ug whole cell lysate)
A:HeLa S3(GTX14654)
B:Hep G2 (GTX27900)
15% SDS PAGE
GTX101487 diluted at 1:5000

GTX101487_39995_WB_M_brain_19011506_143.jpg

GTX101487 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
15% SDS PAGE
GTX101487 diluted at 1:1000

GTX101487_39995_IFA_19011506_414.jpg

GTX101487 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using COX5A(GTX101487) antibody at 1:200 dilution.

GTX101487_39995_20150410_IHC_M_2_19011506_917.jpg

GTX101487 IHC-P Image

COX5A antibody [N1C3] detects COX5A protein at mitochondria on mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
COX5A antibody [N1C3] (GTX101487) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101487_39995_20150410_IHC_M_19011506_593.jpg

GTX101487 IHC-P Image

COX5A antibody [N1C3] detects COX5A protein at mitochondria on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
COX5A antibody [N1C3] (GTX101487) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169