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COX5B antibody

Anti-COX5B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33114
Anti-COX5B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33114
Anti-COX5B antibody used in Immunoprecipitation (IP). GTX33114
Anti-COX5B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33114
Anti-COX5B antibody used in IHC (Paraffin sections) (IHC-P). GTX33114
Anti-COX5B antibody used in Western Blot (WB). GTX33114
Anti-COX5B antibody used in IHC (Paraffin sections) (IHC-P). GTX33114
Anti-COX5B antibody used in IHC (Paraffin sections) (IHC-P). GTX33114

Cat. No. GTX33114

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all COX5B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:100
IP 1:50 - 1:100
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 32-129 of human COX5B (NP_001853.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cytochrome c oxidase subunit 5B , COXVB

Cellular Localization

Mitochondrion inner membrane

Background

Cytochrome C oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Vb of the human mitochondrial respiratory chain enzyme. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-COX5B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33114

GTX33114 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX33114 COX5B antibody.
Blue : DAPI
Dilution : 1:100

Anti-COX5B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33114

GTX33114 ICC/IF Image

ICC/IF analysis of C6 cells using GTX33114 COX5B antibody.
Blue : DAPI
Dilution : 1:100

Anti-COX5B antibody used in Immunoprecipitation (IP). GTX33114

GTX33114 IP Image

IP analysis of HepG2 cell lysate using GTX33114 COX5B antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000

Anti-COX5B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33114

GTX33114 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX33114 COX5B antibody.
Blue : DAPI
Dilution : 1:100

Anti-COX5B antibody used in IHC (Paraffin sections) (IHC-P). GTX33114

GTX33114 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX33114 COX5B antibody.
Dilution : 1:100

Anti-COX5B antibody used in Western Blot (WB). GTX33114

GTX33114 WB Image

WB analysis of various sample lysates using GTX33114 COX5B antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane

Anti-COX5B antibody used in IHC (Paraffin sections) (IHC-P). GTX33114

GTX33114 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX33114 COX5B antibody.
Dilution : 1:100

Anti-COX5B antibody used in IHC (Paraffin sections) (IHC-P). GTX33114

GTX33114 IHC-P Image

IHC-P analysis of human colon tissue using GTX33114 COX5B antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399