APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:200 - 1:2000 |
1:50 - 1:200 |
1:20 - 1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 25-109 of human COX6A1 (NP_004364.2).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
cytochrome c oxidase subunit 6A1 , CMTRID , COX6A , COX6AL
Cellular Localization
Mitochondrion inner membrane
Background
Cytochrome c oxidase (COX), the terminal enzyme of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. It is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in the electron transfer and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes polypeptide 1 (liver isoform) of subunit VIa, and polypeptide 1 is found in all non-muscle tissues. Polypeptide 2 (heart/muscle isoform) of subunit VIa is encoded by a different gene, and is present only in striated muscles. These two polypeptides share 66% amino acid sequence identity. It has been reported that there may be several pseudogenes on chromosomes 1, 6, 7q21, 7q31-32 and 12. However, only one pseudogene (COX6A1P) on chromosome 1p31.1 has been documented. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX64790 WB Image
WB analysis of various sample lysates using GTX64790 COX6A1 antibody. Dilution : 1:1000 Loading : 25μg per lane
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GTX64790 ICC/IF Image
ICC/IF analysis of U2OS cells using GTX64790 COX6A1 antibody. Blue : DAPI Dilution : 1:100
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GTX64790 IHC-P Image
IHC-P analysis of rat cerebellum tissue using GTX64790 COX6A1 antibody. Dilution : 1:100
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GTX64790 IHC-P Image
IHC-P analysis of mouse liver tissue using GTX64790 COX6A1 antibody. Dilution : 1:100
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GTX64790 ICC/IF Image
ICC/IF analysis of NIH/3T3 cells using GTX64790 COX6A1 antibody. Blue : DAPI Dilution : 1:100
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GTX64790 IHC-P Image
IHC-P analysis of human liver cancer tissue using GTX64790 COX6A1 antibody. Dilution : 1:100
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GTX64790 ICC/IF Image
ICC/IF analysis of C6 cells using GTX64790 COX6A1 antibody. Blue : DAPI Dilution : 1:100
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REFERENCE
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REVIEW
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