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COX6A2 antibody [N3C3]

Anti-COX6A2 antibody [N3C3] used in Western Blot (WB). GTX108261
Anti-COX6A2 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX108261
Anti-COX6A2 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108261
Anti-COX6A2 antibody [N3C3] used in Western Blot (WB). GTX108261

Cat. No. GTX108261

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all COX6A2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

11 kDa. ( Note )

Positive Control

Raji

Product Note

Based on sequence homology, this antibody may cross react with COX6A1 protein.

Predict Reactivity

Rat, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.9 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human COX6A2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950022

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cytochrome c oxidase subunit 6A2 , COX6AH , COXVIAH

Cellular Localization

Mitochondrion inner membrane

Background

Cytochrome c oxidase (COX), the terminal enzyme of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. It is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may be involved in the regulation and assembly of the complex. This nuclear gene encodes polypeptide 2 (heart/muscle isoform) of subunit VIa, and polypeptide 2 is present only in striated muscles. Polypeptide 1 (liver isoform) of subunit VIa is encoded by a different gene, and is found in all non-muscle tissues. These two polypeptides share 66% amino acid sequence identity. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-COX6A2 antibody [N3C3] used in Western Blot (WB). GTX108261

GTX108261 WB Image

Sample(30 μg of whole cell lysate)
A:Raji(GTX27908)
12% SDS PAGE
GTX108261 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-COX6A2 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX108261

GTX108261 IHC-P Image

COX6A2 antibody [N3C3] detects COX6A2 protein at mitochondria on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
COX6A2 antibody [N3C3] (GTX108261) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-COX6A2 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108261

GTX108261 ICC/IF Image

COX6A2 antibody [N3C3] detects COX6A2 protein at mitochondria by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min.
Green: COX6A2 protein stained by COX6A2 antibody [N3C3] (GTX108261) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-COX6A2 antibody [N3C3] used in Western Blot (WB). GTX108261

GTX108261 WB Image

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with COX6A2 antibody [N3C3] (GTX108261) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169