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COX6A2 antibody [N3C3]

Anti-COX6A2 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX108261
Anti-COX6A2 antibody [N3C3] used in Western Blot (WB). GTX108261
Anti-COX6A2 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108261

Cat No. GTX108261

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

11 kDa. ( Note )

Positive Control

Raji

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.9 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human COX6A2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cytochrome C Oxidase Subunit 6A2,Cox6Ah,Coxviah,Cox6A2

Cellular Localization

Mitochondrion inner membrane

Background

Cytochrome c oxidase (COX), the terminal enzyme of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. It is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may be involved in the regulation and assembly of the complex. This nuclear gene encodes polypeptide 2 (heart/muscle isoform) of subunit VIa, and polypeptide 2 is present only in striated muscles. Polypeptide 1 (liver isoform) of subunit VIa is encoded by a different gene, and is found in all non-muscle tissues. These two polypeptides share 66% amino acid sequence identity. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-COX6A2 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX108261

GTX108261 IHC-P Image

COX6A2 antibody [N3C3] detects COX6A2 protein at mitochondria on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
COX6A2 antibody [N3C3] (GTX108261) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-COX6A2 antibody [N3C3] used in Western Blot (WB). GTX108261

GTX108261 WB Image

Sample(30 µg of whole cell lysate)
A:Raji(GTX27908)
12% SDS PAGE
GTX108261 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-COX6A2 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108261

GTX108261 ICC/IF Image

COX6A2 antibody [N3C3] detects COX6A2 protein at mitochondria by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min.
Green: COX6A2 protein stained by COX6A2 antibody [N3C3] (GTX108261) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE
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REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169