GeneTex
United States (US)

COX6B1 antibody [N2C3]

GTX108249_39750_IFA_19011507_221.jpg
GTX108249_WB_19011507_461.jpg
GTX108249_39750_IHC_19011507_937.jpg

Cat No. GTX108249

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

10 kDa. ( Note )

Positive Control

A431 , HeLa , HepG2

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human COX6B1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cytochrome C Oxidase Subunit 6B1,Cox6B,Coxg,Coxvib1,Cox6B1

Cellular Localization

Mitochondrion intermembrane space

Background

Cytochrome c oxidase (COX), the terminal enzyme of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. It is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may be involved in the regulation and assembly of the complex. This nuclear gene encodes subunit VIb. Three pseudogenes COX6BP-1, COX6BP-2 and COX6BP-3 have been found on chromosomes 7, 17 and 22q13.1-13.2, respectively. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX108249_39750_IFA_19011507_221.jpg

GTX108249 ICC/IF Image

COX6B1 antibody [N2C3] detects COX6B1 protein at mitochondria by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: COX6B1 protein stained by COX6B1 antibody [N2C3] (GTX108249) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX108249_WB_19011507_461.jpg

GTX108249 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
15% SDS PAGE
COX6B1 antibody
GTX108249 diluted at 1:1000

GTX108249_39750_IHC_19011507_937.jpg

GTX108249 IHC-P Image

Immunohistochemical analysis of paraffin-embedded BCC3 xenograft, using COX6B1(GTX108249) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169