CPF1 antibody

Cat. No. GTX133297

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF
Package	100 μl ($399), 25 μl ($169)

See all CPF1 products

Datasheet
Document

View product citations for antibody GTX133297 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:10000
ICC/IF	1:100-1:1000

Not tested in other applications.

Positive Control

293T , CPF1-transfected 293T (Acidaminococcus sp. BV3L6)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.45 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Acidaminococcus sp. BV3L6

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of Acidaminococcus sp. BV3L6 CPF1 protein. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886918

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

GTX133297 ICC/IF Image

CPF1 antibody detects CPF1 protein at cytoplasm by immunofluorescent analysis.
Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CPF1 protein stained by CPF1 antibody (GTX133297) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX133297 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (5 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CPF1 antibody (GTX133297) diluted at 1:5000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty