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CPI-17 antibody [4H10]

Anti-CPI-17 antibody [4H10] used in Western Blot (WB). GTX53770

Cat. No. GTX53770

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4H10

Isotype

 IgG2a

Application

 WB, FACS, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all CPI-17 products

APPLICATION

Application Note

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1,000. Recommended starting dilution is 1:500.

Calculated MW

17 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human CPI-17 (1-147aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein phosphatase 1 regulatory inhibitor subunit 14A , CPI-17 , CPI17 , PPP1INL

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene belongs to the protein phosphatase 1 (PP1) inhibitor family. This protein is an inhibitor of smooth muscle myosin phosphatase, and has higher inhibitory activity when phosphorylated. Inhibition of myosin phosphatase leads to increased myosin phosphorylation and enhanced smooth muscle contraction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES

Anti-CPI-17 antibody [4H10] used in Western Blot (WB). GTX53770

GTX53770 WB Image

WB analysis of A549 lysate (30ug) using CPI-17 antibody [4H10] at a dilution of 1:500.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399