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CPS1 antibody [GT1274]

Recombinant antibody
Anti-CPS1 antibody [GT1274] used in Western Blot (WB). GTX03186
Anti-CPS1 antibody [GT1274] used in Western Blot (WB). GTX03186
Anti-CPS1 antibody [GT1274] used in IHC (Paraffin sections) (IHC-P). GTX03186
Anti-CPS1 antibody [GT1274] used in IHC (Paraffin sections) (IHC-P). GTX03186

Cat. No. GTX03186

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1274

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($439)
See all CPS1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

165 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.33 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human CPS1.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

carbamoyl-phosphate synthase 1 , CPSASE1 , PHN

Cellular Localization

Mitochondrion , Nucleolus

Background

The mitochondrial enzyme encoded by this gene catalyzes synthesis of carbamoyl phosphate from ammonia and bicarbonate. This reaction is the first committed step of the urea cycle, which is important in the removal of excess urea from cells. The encoded protein may also represent a core mitochondrial nucleoid protein. Three transcript variants encoding different isoforms have been found for this gene. The shortest isoform may not be localized to the mitochondrion. Mutations in this gene have been associated with carbamoyl phosphate synthetase deficiency, susceptibility to persistent pulmonary hypertension, and susceptibility to venoocclusive disease after bone marrow transplantation.[provided by RefSeq, May 2010]

Database

Research Area

DATA IMAGES

Anti-CPS1 antibody [GT1274] used in Western Blot (WB). GTX03186

GTX03186 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CPS1 antibody [GT1274] (GTX03186) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CPS1 antibody [GT1274] used in Western Blot (WB). GTX03186

GTX03186 WB Image

WB analysis of various samples using GTX03186 CPS1 antibody [GT1274].
Dilution : 1:1000
Loading : 25μg per lane

Anti-CPS1 antibody [GT1274] used in IHC (Paraffin sections) (IHC-P). GTX03186

GTX03186 IHC-P Image

IHC-P analysis of human liver tissue section using GTX03186 CPS1 antibody [GT1274].
Dilution : 1:100

Anti-CPS1 antibody [GT1274] used in IHC (Paraffin sections) (IHC-P). GTX03186

GTX03186 IHC-P Image

IHC-P analysis of rat liver tissue section using GTX03186 CPS1 antibody [GT1274].
Blue : DAPI for nuclear staining
Dilution : 1:100

REFERENCE

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REVIEW

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Package List Price ($)
$ 439