GeneTex

  • Country / Location Selection

United States (US)

CPSF2 antibody [N1N3]

Anti-CPSF2 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117730
Anti-CPSF2 antibody [N1N3] used in Western Blot (WB). GTX117730
Anti-CPSF2 antibody [N1N3] used in IHC (Whole mount) (IHC-Wm). GTX117730

Cat. No. GTX117730

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Wm

Reactivity

 Human, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all CPSF2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

Positive Control

U87-MG

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CPSF2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10624663

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cleavage and polyadenylation specific factor 2 , CPSF100

Background

Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Involved in the histone 3' end pre-mRNA processing.

Database

Research Area

DATA IMAGES

Anti-CPSF2 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117730

GTX117730 ICC/IF Image

CPSF2 antibody [N1N3] detects CPSF2 protein at nucleus by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CPSF2 protein stained by CPSF2 antibody [N1N3] (GTX117730) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-CPSF2 antibody [N1N3] used in Western Blot (WB). GTX117730

GTX117730 WB Image

Sample (30 ug of whole cell lysate)
A: U87-MG
7.5% SDS PAGE
GTX117730 diluted at 1:1000

Anti-CPSF2 antibody [N1N3] used in IHC (Whole mount) (IHC-Wm). GTX117730

GTX117730 IHC-Wm Image

Immunohistochemical analysis (whole mount) of zebrafish embryo, using CPSF2 antibody [N1N3] (GTX117730) at 1:200 dilution.

REFERENCE

There are currently no references for CPSF2 antibody [N1N3] (GTX117730). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for CPSF2 antibody [N1N3] (GTX117730). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169