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CPT1A antibody [C1C2], Internal

Anti-CPT1A antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX114337
Anti-CPT1A antibody [C1C2], Internal used in Western Blot (WB). GTX114337
Anti-CPT1A antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX114337

Cat No. GTX114337

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

Positive Control

A549 , HeLa , HepG2

Predict Reactivity

Rat, Sheep, Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CPT1A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619309

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

carnitine palmitoyltransferase 1A , CPT1 , CPT1-L , L-CPT1

Background

The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CPT1A antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX114337

GTX114337 IHC-P Image

CPT1A antibody [C1C2], Internal detects CPT1A protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
CPT1A stained by CPT1A antibody [C1C2], Internal (GTX114337) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CPT1A antibody [C1C2], Internal used in Western Blot (WB). GTX114337

GTX114337 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CPT1A antibody [C1C2], Internal (GTX114337) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CPT1A antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX114337

GTX114337 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MDA-MB157 xenograft, using CPT1A(GTX114337) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

CPT1A antibody [C1C2], Internal Cat No. GTX114337

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 07-Sep-2021
Score :
Application Tested : WB
Sample : mouse liver tissue lysate
Amount used : 20μg
Blocking : 5% non-fat milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, > 12Hr
Lane Description :
Lane 1-6 : control
Lane 7-12 : treatment
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Cancer Metab in 2014. PMID: 25225614
Application Reference
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169