GeneTex

  • Country / Location Selection

United States (US)

CPT1A antibody [C1C2], Internal

Anti-CPT1A antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX114337
Anti-CPT1A antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX114337
Anti-CPT1A antibody [C1C2], Internal used in Western Blot (WB). GTX114337
Anti-CPT1A antibody [C1C2], Internal used in Western Blot (WB). GTX114337

Cat. No. GTX114337

Reference 12
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all CPT1A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

Predict Reactivity

Rat, Sheep, Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CPT1A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619309

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

carnitine palmitoyltransferase 1A , CPT1 , CPT1-L , L-CPT1

Background

The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CPT1A antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX114337

GTX114337 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MDA-MB157 xenograft, using CPT1A(GTX114337) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CPT1A antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX114337

GTX114337 IHC-P Image

CPT1A antibody [C1C2], Internal detects CPT1A protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
CPT1A stained by CPT1A antibody [C1C2], Internal (GTX114337) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CPT1A antibody [C1C2], Internal used in Western Blot (WB). GTX114337

GTX114337 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CPT1A antibody [C1C2], Internal (GTX114337) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CPT1A antibody [C1C2], Internal used in Western Blot (WB). GTX114337

GTX114337 WB Image

The data was published in the journal Cancer Metab in 2014. PMID: 25225614

REFERENCE

Application Reference

Ngo J et al. EMBO J 2023; 42 (11) : e111901 Mitochondrial morphology controls fatty acid utilization by changing CPT1 sensitivity to malonyl-CoA.
Zhu Z et al. Evid Based Complement Alternat Med 2021; 2021 : 9931542 Quantitative Profiling of Oxylipin Reveals the Mechanism of Pien-Tze-Huang on Alcoholic Liver Disease.
Application : WB
Ezzeddini R et al. J Physiol Biochem 2021; 77 (2) : 249-260 Downregulation of fatty acid oxidation by involvement of HIF-1α and PPARγ in human gastric adenocarcinoma and related clinical significance.
Application : WB
Chang TC et al. Pharmacol Res 2021; 163 : 105298 Ugonin J improves metabolic disorder and ameliorates nonalcoholic fatty liver disease by regulating the AMPK/AKT signaling pathway.
Application : WB
Reactivity : Human
Tsai YT et al. Nutrients 2020; 12 (3) Antrodia cinnamomea Confers Obesity Resistance and Restores Intestinal Barrier Integrity in Leptin-deficient Obese Mice.
Show More +
Submit a Reference

REVIEW

CPT1A antibody [C1C2], Internal Cat. No. GTX114337

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-CPT1A antibody [C1C2], Internal used in Western Blot (WB). GTX114337
Date : Anonymous submitted on 07-Sep-2021
Score :
Application Tested : WB
Sample : mouse liver tissue lysate
Amount used : 20μg
Blocking : 5% non-fat milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, > 12Hr
Lane Description :
Lane 1-6 : control
Lane 7-12 : treatment
Submit a Review
Application Reference
Ngo J et al. EMBO J 2023; 42 (11):e111901 Mitochondrial morphology controls fatty acid utilization by changing CPT1 sensitivity to malonyl-CoA.
Zhu Z et al. Evid Based Complement Alternat Med 2021; 2021:9931542 Quantitative Profiling of Oxylipin Reveals the Mechanism of Pien-Tze-Huang on Alcoholic Liver Disease.
Application : WB
Ezzeddini R et al. J Physiol Biochem 2021; 77 (2):249-260 Downregulation of fatty acid oxidation by involvement of HIF-1α and PPARγ in human gastric adenocarcinoma and related clinical significance.
Application : WB
Chang TC et al. Pharmacol Res 2021; 163:105298 Ugonin J improves metabolic disorder and ameliorates nonalcoholic fatty liver disease by regulating the AMPK/AKT signaling pathway.
Application : WB
Reactivity : Human
Tsai YT et al. Nutrients 2020; 12 (3) Antrodia cinnamomea Confers Obesity Resistance and Restores Intestinal Barrier Integrity in Leptin-deficient Obese Mice.
Chang TC et al. BMC Complement Altern Med 2019; 19 (1):368 Helminthostachys zeylanica alleviates hepatic steatosis and insulin resistance in diet-induced obese mice.
Application : WB
Reactivity : Human
You BJ et al. Sci Rep 2019; 9 (1):3306 Fenofibrate induces human hepatoma Hep3B cells apoptosis and necroptosis through inhibition of thioesterase domain of fatty acid synthase.
Application : WB
Reactivity : Human
You BJ et al. Chem Res Toxicol 2019; 32 (2):255-264
Application : WB
Reactivity : Human
Tarling EJ et al. Arterioscler Thromb Vasc Biol 2015; (Epub) The Nuclear Receptor FXR Uncouples the Actions of miR-33 From SREBP-2.
Application : WB
Roy D et al. Cancer Metab 2014; 2:13 Loss of HSulf-1 promotes altered lipid metabolism in ovarian cancer.
Application : WB
Reactivity : Human
Setoyama D et al. Genes Cells 2013; 18 (12):1107-19 Metabolomics reveals that carnitine palmitoyltransferase-1 is a novel target for oxidative inactivation in human cells.
Application : WB
Reactivity : Human
Jia X et al. Life Sci 2012; 90 (23-24):934-43 The modulation of hepatic adenosine triphosphate and inflammation by eicosapentaenoic acid during severe fibrotic progression in the SHRSP5/Dmcr rat model.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169