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CPT1A antibody [HL1164]

Anti-CPT1A antibody [HL1164] used in Western Blot (WB). GTX636468
Anti-CPT1A antibody [HL1164] used in Western Blot (WB). GTX636468
Anti-CPT1A antibody [HL1164] used in Western Blot (WB). GTX636468
Anti-CPT1A antibody [HL1164] used in IHC (Paraffin sections) (IHC-P). GTX636468

Cat No. GTX636468

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1164

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CPT1A. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909987

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

carnitine palmitoyltransferase 1A , CPT1 , CPT1-L , L-CPT1

Cellular Localization

Mitochondrion outer membrane

Background

The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CPT1A antibody [HL1164] used in Western Blot (WB). GTX636468

GTX636468 WB Image

Wild-type (WT) and CPT1A knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CPT1A antibody [HL1164] (GTX636468) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-CPT1A antibody [HL1164] used in Western Blot (WB). GTX636468

GTX636468 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CPT1A antibody [HL1164] (GTX636468) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CPT1A antibody [HL1164] used in Western Blot (WB). GTX636468

GTX636468 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CPT1A antibody [HL1164] (GTX636468) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CPT1A antibody [HL1164] used in IHC (Paraffin sections) (IHC-P). GTX636468

GTX636468 IHC-P Image

CPT1A antibody [HL1164] detects CPT1A protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human kidney.
CPT1A stained by CPT1A antibody [HL1164] (GTX636468) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

There are currently no references for CPT1A antibody [HL1164] (GTX636468). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for CPT1A antibody [HL1164] (GTX636468). Be the first to share your experience with this product.
Package List Price ($)
$ 379
$ 169
Custom Conjugations