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CPT1A antibody, Internal

Anti-CPT1A antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89237
Anti-CPT1A antibody, Internal used in Western Blot (WB). GTX89237
Anti-CPT1A antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89237

Cat. No. GTX89237

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all CPT1A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.3μg/ml
IHC-P 4μg/ml

Note :

IHC-P
Human Kidney shows mitochindrial staining in PCT.
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89237-PEP).

Product Note

This antibody is expected to recognize both reported isoforms (NP_001867.2; NP_001027017.1) but is not expected to recognise CPT1B.

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-DPAQTVEQRLKLFK, from the internal region of the protein sequence according to NP_001867.2; NP_001027017.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

carnitine palmitoyltransferase 1A , CPT1 , CPT1-L , L-CPT1

Cellular Localization

Mitochondrion outer membrane

Background

The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CPT1A antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89237

GTX89237 IHC-P Image

IHC-P analysis of human heart using GTX89237 CPT1A antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 4μg/ml

Anti-CPT1A antibody, Internal used in Western Blot (WB). GTX89237

GTX89237 WB Image

WB analysis of human liver lysate using GTX89237 CPT1A antibody, Internal.
Dilution : 0.1μg/ml
Loading : 35μg protein in RIPA buffer

Anti-CPT1A antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89237

GTX89237 IHC-P Image

IHC-P analysis of human kidney using GTX89237 CPT1A antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 4μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399