APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 0.1-0.3μg/ml |
| 4μg/ml |
|
Note :
IHC-P
Human Kidney shows mitochindrial staining in PCT.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89237-PEP).
Product Note
This antibody is expected to recognize both reported isoforms (NP_001867.2; NP_001027017.1) but is not expected to recognise CPT1B.
PROPERTIES
Form
Liquid
Buffer
TBS pH7.3, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-DPAQTVEQRLKLFK, from the internal region of the protein sequence according to NP_001867.2; NP_001027017.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
carnitine palmitoyltransferase 1A , CPT1 , CPT1-L , L-CPT1
Cellular Localization
Mitochondrion outer membrane
Background
The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
|
GTX89237 IHC-P Image
IHC-P analysis of human kidney using GTX89237 CPT1A antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 4μg/ml
|
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|
GTX89237 WB Image
WB analysis of human liver lysate using GTX89237 CPT1A antibody, Internal.
Dilution : 0.1μg/ml
Loading : 35μg protein in RIPA buffer
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GTX89237 IHC-P Image
IHC-P analysis of human heart using GTX89237 CPT1A antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 4μg/ml
|
REFERENCE
There are currently no references for CPT1A antibody, Internal (GTX89237). Be the first to share your publications with this product.
REVIEW
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