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CPT1B antibody [C1C2], Internal

Anti-CPT1B antibody [C1C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117231
Anti-CPT1B antibody [C1C2], Internal used in Western Blot (WB). GTX117231

Cat. No. GTX117231

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all CPT1B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Sheep, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CPT1B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10722705

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

carnitine palmitoyltransferase 1B , CPT1-M , CPT1M , CPTI , CPTI-M , M-CPT1 , MCCPT1 , MCPT1

Cellular Localization

Mitochondrion outer membrane

Background

The protein encoded by this gene, a member of the carnitine/choline acetyltransferase family, is the rate-controlling enzyme of the long-chain fatty acid beta-oxidation pathway in muscle mitochondria. This enzyme is required for the net transport of long-chain fatty acyl-CoAs from the cytoplasm into the mitochondria. Multiple transcript variants encoding different isoforms have been found for this gene, and one of the transcripts is bicistronic. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CPT1B antibody [C1C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117231

GTX117231 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using CPT1B(GTX117231) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-CPT1B antibody [C1C2], Internal used in Western Blot (WB). GTX117231

GTX117231 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa
7.5% SDS PAGE
GTX117231 diluted at 1:1000

REFERENCE

Application Reference

Chen W et al. BMC Biol 2023; 21 (1) : 27 Cold exposure alters lipid metabolism of skeletal muscle through HIF-1α-induced mitophagy.
Dang Y et al. Biomed Pharmacother 2020; 127 : 110127 Gan-Jiang-Ling-Zhu decoction alleviates hepatic steatosis in rats by the miR-138-5p/CPT1B axis.
Application : WB
Reactivity : Rat
Wen SY et al. J Cell Physiol 2017; (Epub) High density lipoprotein (HDL) reverses palmitic acid induced energy metabolism imbalance by switching CD36 and GLUT4 signaling pathways in cardiomyocyte.
Application : WB
Reactivity : Rat
Zhang Y et al. Obesity (Silver Spring) 2016; (Epub) Cardiac-specific down-regulation of carnitine palmitoyltransferase-1b (CPT-1b) prevents cardiac remodeling in obese mice.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169