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CPT1B antibody

Anti-CPT1B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64535
Anti-CPT1B antibody used in Western Blot (WB). GTX64535
Anti-CPT1B antibody used in IHC (Paraffin sections) (IHC-P). GTX64535
Anti-CPT1B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64535
Anti-CPT1B antibody used in IHC (Paraffin sections) (IHC-P). GTX64535
Anti-CPT1B antibody used in IHC (Paraffin sections) (IHC-P). GTX64535

Cat. No. GTX64535

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all CPT1B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 533-772 of human CPT1B (NP_004368.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2888602

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

carnitine palmitoyltransferase 1B , CPT1-M , CPT1M , CPTI , CPTI-M , M-CPT1 , MCCPT1 , MCPT1

Background

The protein encoded by this gene, a member of the carnitine/choline acetyltransferase family, is the rate-controlling enzyme of the long-chain fatty acid beta-oxidation pathway in muscle mitochondria. This enzyme is required for the net transport of long-chain fatty acyl-CoAs from the cytoplasm into the mitochondria. Multiple transcript variants encoding different isoforms have been found for this gene, and read-through transcripts are expressed from the upstream locus that include exons from this gene. [provided by RefSeq, Jun 2009]

Database

Research Area

DATA IMAGES

Anti-CPT1B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64535

GTX64535 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX64535 CPT1B antibody.
Blue : DAPI
Dilution : 1:100

Anti-CPT1B antibody used in Western Blot (WB). GTX64535

GTX64535 WB Image

WB analysis of various sample lysates using GTX64535 CPT1B antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-CPT1B antibody used in IHC (Paraffin sections) (IHC-P). GTX64535

GTX64535 IHC-P Image

IHC-P analysis of mouse heart tissue using GTX64535 CPT1B antibody.
Dilution : 1:100

Anti-CPT1B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64535

GTX64535 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX64535 CPT1B antibody.
Blue : DAPI
Dilution : 1:100

Anti-CPT1B antibody used in IHC (Paraffin sections) (IHC-P). GTX64535

GTX64535 IHC-P Image

IHC-P analysis of rat liver tissue using GTX64535 CPT1B antibody.
Dilution : 1:100

Anti-CPT1B antibody used in IHC (Paraffin sections) (IHC-P). GTX64535

GTX64535 IHC-P Image

IHC-P analysis of human colon tissue using GTX64535 CPT1B antibody.
Dilution : 1:100

REFERENCE

Application Reference

Chen YP et al. J Cell Biochem 2017; (Epub) Acute hypoxic preconditioning prevents palmitic acid-induced cardiomyocyte apoptosis via switching metabolic GLUT4-glucose pathway back to CD36-fatty acid dependent.
Application : WB
Reactivity : Rat
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399