Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CPT2 antibody [N1C1] (GTX104115) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample(30 μg of whole cell lysate)
A:HeLa S3(GTX14654)
7.5% SDS PAGE
GTX104115 diluted at 1:1000