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CRABP2 antibody [004]

Anti-CRABP2 antibody [004] used in Immunoprecipitation (IP). GTX02001
Anti-CRABP2 antibody [004] used in Western Blot (WB). GTX02001
Anti-CRABP2 antibody [004] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02001
Anti-CRABP2 antibody [004] used in Flow cytometry (FACS). GTX02001

Cat No. GTX02001

Host

Rabbit

Clonality

Monoclonal

Clone Name

004

Isotype

IgG

Application

WB, ICC/IF, FACS, IP

Reactivity

Human
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF Assay dependent
FACS Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.2 μm filtered PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant Human CRABP2 protein

Purification

Purified by Protein A

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cellular retinoic acid binding protein 2 , CRABP-II , RBP6

Cellular Localization

Cytoplasm , Endoplasmic reticulum , Nucleus

Background

This gene encodes a member of the retinoic acid (RA, a form of vitamin A) binding protein family and lipocalin/cytosolic fatty-acid binding protein family. The protein is a cytosol-to-nuclear shuttling protein, which facilitates RA binding to its cognate receptor complex and transfer to the nucleus. It is involved in the retinoid signaling pathway, and is associated with increased circulating low-density lipoprotein cholesterol. Alternatively spliced transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Dec 2010]

Database

Research Area

DATA IMAGES

Anti-CRABP2 antibody [004] used in Immunoprecipitation (IP). GTX02001

GTX02001 IP Image

IP analysis of 293T whole cell lysate using GTX02001 CRABP2 antibody [004].
IP antibody : 0.5 μl per 0.5 mg total whole cell lysate
Dilution : 1:500
Immunomagnetic beads Protein G : 60 μg

Anti-CRABP2 antibody [004] used in Western Blot (WB). GTX02001

GTX02001 WB Image

WB analysis of various samples using GTX02001 CRABP2 antibody [004].
Lane A : 293T whole cell lysate
Lane B : MCF-7 whole cell lysate
Dilution : 1:500
Loading : 30 μg

Anti-CRABP2 antibody [004] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02001

GTX02001 ICC/IF Image

ICC/IF analysis of PFA-fixed MCF-7 cells using GTX02001 CRABP2 antibody [004].
Green : Primary antibody
Blue : DAPI
Dilution : 1:300
Permeabilzation : 1% Triton X-100

Anti-CRABP2 antibody [004] used in Flow cytometry (FACS). GTX02001

GTX02001 FACS Image

FACS analysis of MCF-7 cells using GTX02001 CRABP2 antibody [004].
The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

REFERENCE

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REVIEW

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