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CRABP2 antibody [AT2E11]

Anti-CRABP2 antibody [AT2E11] used in Western Blot (WB). GTX50029

Cat No. GTX50029

Host

Mouse

Clonality

Monoclonal

Clone Name

AT2E11

Isotype

IgG2a

Application

WB, ICC/IF, FACS, ELISA

Reactivity

Human, Mouse
Package
100 μl ($319)

APPLICATION

Application Note

We recommend the following starting dilutions:
Western Blot: Use at 1:250 ~ 1000.

Optimal working concentrations should be determined experimentally by the end user.

Calculated MW

16 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human CRABP2 (1-138aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Cellular Retinoic Acid Binding Protein 2 , Crabp-Ii , Rbp6 , Crabp2

Cellular Localization

Upon ligand binding, a conformation change exposes a nuclear localization motif and the protein is transported into the nucleus.

Background

The cellular retinoic acid-binding protein II (CRABP-II) is involved in the conversion of vitamin A into its intracellular active form retinoic acid, which regulate the genes responsible for lipid metabolism and adipocyte differentiation. CRABP2 gene is located on chromosome 1q21-23 and this region has been linked with related disorders such as familial combined hyperlipidemia (FCHL) and type 2 diabetes mellitus.

Database

Research Area

DATA IMAGES

Anti-CRABP2 antibody [AT2E11] used in Western Blot (WB). GTX50029

GTX50029 WB Image

Cell lysates of mouse eye (60ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CRABP2 (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 319