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CRABP2 antibody [AT2E11]

Anti-CRABP2 antibody [AT2E11] used in Western Blot (WB). GTX50029

Cat No. GTX50029

Host

Mouse

Clonality

Monoclonal

Clone Name

AT2E11

Isotype

IgG2a

Application

WB, ICC/IF, FACS, ELISA

Reactivity

Human, Mouse
Package
100 μl ($339)

APPLICATION

Application Note

We recommend the following starting dilutions:
Western Blot: Use at 1:250 ~ 1000.

Optimal working concentrations should be determined experimentally by the end user.

Calculated MW

16 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human CRABP2 (1-138aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cellular retinoic acid binding protein 2 , CRABP-II , RBP6

Cellular Localization

Cytoplasm , Endoplasmic reticulum , Nucleus

Background

This gene encodes a member of the retinoic acid (RA, a form of vitamin A) binding protein family and lipocalin/cytosolic fatty-acid binding protein family. The protein is a cytosol-to-nuclear shuttling protein, which facilitates RA binding to its cognate receptor complex and transfer to the nucleus. It is involved in the retinoid signaling pathway, and is associated with increased circulating low-density lipoprotein cholesterol. Alternatively spliced transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Dec 2010]

Database

Research Area

DATA IMAGES

Anti-CRABP2 antibody [AT2E11] used in Western Blot (WB). GTX50029

GTX50029 WB Image

Cell lysates of mouse eye (60ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CRABP2 (1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf