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CRABP2 antibody [N1C3]

Anti-CRABP2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101551
Anti-CRABP2 antibody [N1C3] used in Western Blot (WB). GTX101551
Anti-CRABP2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101551
Anti-CRABP2 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101551
Anti-CRABP2 antibody [N1C3] used in Western Blot (WB). GTX101551

Cat. No. GTX101551

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all CRABP2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

Positive Control

HepG2 , mouse brain , mouse fetal brain , rat brain

Predict Reactivity

Bovine, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CRABP2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618785

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cellular retinoic acid binding protein 2 , CRABP-II , RBP6

Background

A number of specific carrier proteins for members of the vitamin A family have been discovered. Cellular retinoic acid binding proteins (CRABP) are low molecular weight proteins whose precise function remains unknown. The inducibility of the CRABP2 gene suggests that this isoform is important in retinoic acid-mediated regulation of human skin growth and differentiation. It has been postulated that the CRABP2 gene is transcriptionally regulated by a newly synthesized regulatory protein. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CRABP2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101551

GTX101551 IHC-P Image

CRABP2 antibody [N1C3] detects CRABP2 protein expression by immunohistochemical analysis.
Sample:Paraffin-Embedded adult mouse retina.
Green: CRABP2 protein stained by CRABP2 antibody [N1C3] (GTX101551) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-CRABP2 antibody [N1C3] used in Western Blot (WB). GTX101551

GTX101551 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
15% SDS PAGE
GTX101551diluted at 1:1000

Anti-CRABP2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX101551

GTX101551 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MDAMB468 xenograft, using CRABP2(GTX101551) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CRABP2 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101551

GTX101551 ICC/IF Image

CRABP2 antibody [N1C3] detects CRABP2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CRABP2 protein stained by CRABP2 antibody [N1C3] (GTX101551) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-CRABP2 antibody [N1C3] used in Western Blot (WB). GTX101551

GTX101551 WB Image

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with CRABP2 antibody [N1C3] (GTX101551) diluted at 1:1000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169