GeneTex
United States (US)

CRABP2 antibody [N1C3]

GTX101551_40268_IFA_19011506_682.jpg
GTX101551_40268_20161110_WB_M_R_19011506_144.jpg
GTX101551_WB_19011506_720.jpg
GTX101551_40268_IHC_19011506_157.jpg
GTX101551_40268_20170522_IHC-P_M_19011506_353.jpg

Cat No. GTX101551

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

Positive Control

A549 , HepG2 , mouse brain , mouse fetal brain , rat brain

Predict Reactivity

Bovine, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CRABP2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cellular Retinoic Acid Binding Protein 2,Crabp-Ii,Rbp6,Crabp2

Background

A number of specific carrier proteins for members of the vitamin A family have been discovered. Cellular retinoic acid binding proteins (CRABP) are low molecular weight proteins whose precise function remains unknown. The inducibility of the CRABP2 gene suggests that this isoform is important in retinoic acid-mediated regulation of human skin growth and differentiation. It has been postulated that the CRABP2 gene is transcriptionally regulated by a newly synthesized regulatory protein. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101551_40268_IFA_19011506_682.jpg

GTX101551 ICC/IF Image

CRABP2 antibody [N1C3] detects CRABP2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CRABP2 protein stained by CRABP2 antibody [N1C3] (GTX101551) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX101551_40268_20161110_WB_M_R_19011506_144.jpg

GTX101551 WB Image

Various tissue extracts (50 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with CRABP2 antibody [N1C3] (GTX101551) diluted at 1:1000.

GTX101551_WB_19011506_720.jpg

GTX101551 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
15% SDS PAGE
GTX101551diluted at 1:1000

GTX101551_40268_IHC_19011506_157.jpg

GTX101551 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MDAMB468 xenograft, using CRABP2(GTX101551) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101551_40268_20170522_IHC-P_M_19011506_353.jpg

GTX101551 IHC-P Image

CRABP2 antibody [N1C3] detects CRABP2 protein expression by immunohistochemical analysis.
Sample:Paraffin-Embedded adult mouse retina.
Green: CRABP2 protein stained by CRABP2 antibody [N1C3] (GTX101551) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169