CREB antibody [5G3]

Cat. No. GTX60431

Host	Mouse
Clonality	Monoclonal
Clone Name	5G3
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, ELISA
Reactivity	Human, Mouse, Rat, Monkey
Package	100 μl ($409)

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Datasheet
Document

View product citations for antibody GTX60431 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1/500 - 1/2000
ICC/IF	1/200 - 1/1000
IHC-P	1/200 - 1/1000
ELISA	1/10000

Not tested in other applications.

Calculated MW

37 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human CREB1 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cAMP responsive element binding protein 1 , CREB , CREB-1

Cellular Localization

Nucleus

Background

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Database

Gene ID: 1385 CREB1
UniProt: P16220 CREB1

Research Area

DATA IMAGES

GTX60431 WB Image

WB analysis of K562 (1), Jurkat (2), L1210 (3), HEK293 (4), A431 (5), HeLa (6), Cos7 (7), PC-12 (8), and NIH3T3 (9) cell lysate using GTX60431 CREB antibody [5G3].

GTX60431 ELISA Image

ELISA analysis of antigen using GTX60431 CREB antibody [5G3].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng

GTX60431 IHC-P Image

IHC-P analysis of prostate cancer tissue (left) and submaxillary tumor tissue (right) using GTX60431 CREB antibody [5G3].

GTX60431 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX60431 CREB antibody [5G3].
Green : CREB
Red: Actin filaments

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 409

Qty