CREB antibody [LB9]

ICC/IF analysis of MDA-MB-231 cells using GTX15731 CREB antibody [LB9]. Panel e shows the no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 5 μg/ml in 0.1% BSA and incubated for 3 hours at room temperature

IHC-P analysis of human tonsil tissue using GTX15731 CREB antibody [LB9].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100

WB analysis of 25 ug of various whole cell lysates using GTX15731 CREB antibody [LB9].
Dilution : 1:500

WB analysis of nuclear extracts (30 μg lysate) of Jurkat (Lane 1), HeLa (Lane 2), K-562 (Lane 3), Hep G2 (Lane 4), NIH/3T3 (Lane 5), C2C12 (Lane 6), U-2 OS (Lane 7), A549 (Lane 8), MDA-MB-231 (Lane 9) and T98G (Lane10) using GTX15731 CREB antibody [LB9].
Dilution : 1:500

ChIP analysis of 2 million HeLa cells using GTX15731 CREB antibody [LB9]. Normal Rabbit IgG was used as a negative IP control. The precipitated DNA was detected by PCR with primer set targeting to promoter of NR4A3, CYR61 gene used as positive control target, and the MYOD, SAT2 used as negative control target.

IHC-P analysis of human bladder and rectal tissue using GTX15731 CREB antibody [LB9].
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer for 20 minutes.
Dilution : 1:200

ICC/IF analysis of HeLa cells using GTX15731 CREB antibody [LB9].
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 15 minutes at room temperature
Dilution : 1:400 for at least 1 hour at roomtemperature

ICC/IF analysis of HeLa cells using GTX15731 CREB antibody [LB9].
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 15 minutes at room temperature
Dilution : 1:500 for at least 1 hour at room temperature