GeneTex
United States (US)

CREB antibody [LB9]

GTX15731_1033_IHC-P_19011519_985.jpg
GTX15731_1034_IHC-P_19011519_304.jpg
GTX15731_4_ChIP_19011519_782.jpg
GTX15731_290_ICC-IF_19011519_712.jpg
GTX15731_1518_WB_19011519_556.jpg
GTX15731_289_ICC-IF_19011519_171.jpg
GTX15731_1517_WB_19011519_922.jpg
GTX15731_288_ICC-IF_19011519_184.jpg

Cat No. GTX15731

Host Mouse
Clonality Monoclonal
Clone Name LB9
Isotype IgG1
Application WB, ICC/IF, IHC-P, IP, ELISA, ChIP assay, IHC
Reactivity Human, Mouse, Rat, Primate
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500
ICC/IF 5 μg/ml
IHC-P 1:200
IP 2 μg
ELISA 0.1-1 μg/ml
ChIP assay 2.5 μg/10⁶ cells
IHC Assay dependent
Not tested in other applications.

Calculated MW

37 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS, 1mg/ml BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human CREB-A protein.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Camp Responsive Element Binding Protein 1,Creb,Creb-1,Creb1

Cellular Localization

Nucleus

Background

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016]

Database

Research Area

DATA IMAGES
GTX15731_1033_IHC-P_19011519_985.jpg

GTX15731 IHC-P Image

IHC-P analysis of human bladder and rectal tissue using GTX15731 CREB antibody [LB9].
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer for 20 minutes.
Dilution : 1:200

GTX15731_1034_IHC-P_19011519_304.jpg

GTX15731 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX15731 CREB antibody [LB9].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100

GTX15731_4_ChIP_19011519_782.jpg

GTX15731 ChIP assay Image

ChIP analysis of 2 million HeLa cells using GTX15731 CREB antibody [LB9]. Normal Rabbit IgG was used as a negative IP control. The precipitated DNA was detected by PCR with primer set targeting to promoter of NR4A3, CYR61 gene used as positive control target, and the MYOD, SAT2 used as negative control target.

GTX15731_290_ICC-IF_19011519_712.jpg

GTX15731 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX15731 CREB antibody [LB9].
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 15 minutes at room temperature
Dilution : 1:500 for at least 1 hour at room temperature

GTX15731_1518_WB_19011519_556.jpg

GTX15731 WB Image

WB analysis of nuclear extracts (30 µg lysate) of Jurkat (Lane 1), HeLa (Lane 2), K-562 (Lane 3), Hep G2 (Lane 4), NIH/3T3 (Lane 5), C2C12 (Lane 6), U-2 OS (Lane 7), A549 (Lane 8), MDA-MB-231 (Lane 9) and T98G (Lane10) using GTX15731 CREB antibody [LB9].
Dilution : 1:500

GTX15731_289_ICC-IF_19011519_171.jpg

GTX15731 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX15731 CREB antibody [LB9].
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 15 minutes at room temperature
Dilution : 1:400 for at least 1 hour at roomtemperature

GTX15731_1517_WB_19011519_922.jpg

GTX15731 WB Image

WB analysis of 25 ug of various whole cell lysates using GTX15731 CREB antibody [LB9].
Dilution : 1:500

GTX15731_288_ICC-IF_19011519_184.jpg

GTX15731 ICC/IF Image

ICC/IF analysis of MDA-MB-231 cells using GTX15731 CREB antibody [LB9]. Panel e shows the no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 5 µg/ml in 0.1% BSA and incubated for 3 hours at room temperature