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CREB antibody

Anti-CREB antibody used in Western Blot (WB). GTX112846
Anti-CREB antibody used in IHC (Paraffin sections) (IHC-P). GTX112846
Anti-CREB antibody used in IHC (Frozen sections) (IHC-Fr). GTX112846
Anti-CREB antibody used in IHC (Paraffin sections) (IHC-P). GTX112846
Anti-CREB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112846
Anti-CREB antibody used in IHC (Paraffin sections) (IHC-P). GTX112846
Anti-CREB antibody used in Western Blot (WB). GTX112846
Anti-CREB antibody used in IHC (Paraffin sections) (IHC-P). GTX112846
Anti-CREB antibody used in IHC (Paraffin sections) (IHC-P). GTX112846

Cat No. GTX112846

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

Jurkat , Jurkat nuclear extract , *human CREB1-transfected 293T

Predict Reactivity

Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.15 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CREB. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CREB1 Antibody, cAMP responsive element binding protein 1 Antibody

Cellular Localization

Nucleus

Background

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq]

Research Area

DATA IMAGES
Anti-CREB antibody used in Western Blot (WB). GTX112846

GTX112846 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CREB antibody (GTX112846) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-CREB antibody used in IHC (Paraffin sections) (IHC-P). GTX112846

GTX112846 IHC-P Image

CREB antibody detects CREB protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
CREB antibody (GTX112846) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CREB antibody used in IHC (Frozen sections) (IHC-Fr). GTX112846

GTX112846 IHC-Fr Image

CREB antibody detects CREB protein expression by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: CREB protein stained by CREB antibody (GTX112846) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-CREB antibody used in IHC (Paraffin sections) (IHC-P). GTX112846

GTX112846 IHC-P Image

CREB antibody detects CREB protein at nucleus on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
CREB antibody (GTX112846) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CREB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112846

GTX112846 ICC/IF Image

CREB antibody detects CREB protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CREB protein stained by CREB antibody (GTX112846) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.

Anti-CREB antibody used in IHC (Paraffin sections) (IHC-P). GTX112846

GTX112846 IHC-P Image

CREB antibody detects CREB protein at nucleus in rat testis by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
CREB antibody (GTX112846) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CREB antibody used in Western Blot (WB). GTX112846

GTX112846 WB Image

Jurkat whole cell and nuclear extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CREB antibody (GTX112846) diluted at 1:1000.

Anti-CREB antibody used in IHC (Paraffin sections) (IHC-P). GTX112846

GTX112846 IHC-P Image

CREB antibody detects CREB protein at nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
CREB antibody (GTX112846) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CREB antibody used in IHC (Paraffin sections) (IHC-P). GTX112846

GTX112846 IHC-P Image

CREB antibody detects CREB protein at nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
CREB antibody (GTX112846) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
REVIEW

CREB antibody Cat No. GTX112846

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 13-Mar-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : human CREB1-transfected 293T cells
Amount used : 1*10^5 cells
Blocking : 5% milk in TBST, 25°C, 2Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description : Lane1: 293T cells transfected with pCDNA3.1-myc-human CREB. Lane2: 293T cells transfected with pCDNA3.1.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169