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CREB antibody

Anti-CREB antibody used in Western Blot (WB). GTX27540

Cat No. GTX27540

Host Rabbit
Clonality Polyclonal
Application WB, ELISA, GSA
Reactivity Human, Mouse, Rat
Package
100 μl ($339)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
ELISA 1:5000 - 1:25000
GSA 0.5-1.0 uL per assay
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Specificity/Sensitivity

A partial cross-reactivity is observed against CREM-1 protein.
PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.56 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

CREB-1 (p43) peptide corresponding to a region near the N-terminus of the human protein, conjugated to Keyhole Limpet Hemocyanin (KLH).

Purification

Antiserum
This product was prepared from monospecific antiserum by delipidation and immunoadsorption against an E.coli lysate immobilized on agarose beads.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Camp Responsive Element Binding Protein 1,Creb,Creb-1,Creb1

Background

cyclic AMP responsive element binding protein (CREB) is a ubiquitous transcription factor regulated by changes in cyclic AMP (cAMP) levels. CREB binds as a homodimer to CRE sites (cAMP response elements) and belongs to the basic leucine zipper family of DNA binding proteins. Increases in cAMP levels result in the specific phosphorylation of CREB by protein kinase A. Phosphorylated CREB interacts with the transcriptional co-activator CBP (CREB binding protein or p265). It has recently been demonstrated that CREB phosphorylation correlates with light regulated circadian rhythms.

Database

Research Area

DATA IMAGES
Anti-CREB antibody used in Western Blot (WB). GTX27540

GTX27540 WB Image

Anti-CREB is shown to detect CREB-1 present in Raji B cell nuclear extract. Detection occurs using a 1:1,000 dilution of antibody followed by a 1:5,000 dilution of HRP Goat anti Rabbit IgG with visualization via ECL

REFERENCE
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Package List Price ($)
$ 339