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CREB antibody

Anti-CREB antibody used in Western Blot (WB). GTX27540
Anti-CREB antibody used in Western Blot (WB). GTX27540

Cat. No. GTX27540

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA, ChIP assay, EMSA

Reactivity

 Human, Mouse, Rat
Package
100 μl ($589)
See all CREB products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ELISA 1:5000-1:25000
ChIP assay Assay dependent
EMSA 0.5-1 μL per assay
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Product Note

A partial cross-reactivity is observed against CREM-1 protein.

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.56 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

CREB-1 (p43) peptide corresponding to a region near the N-terminus of the human protein, conjugated to KLH.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cAMP responsive element binding protein 1 , CREB , CREB-1

Cellular Localization

Nucleus

Background

cyclic AMP responsive element binding protein (CREB) is a ubiquitous transcription factor regulated by changes in cyclic AMP (cAMP) levels. CREB binds as a homodimer to CRE sites (cAMP response elements) and belongs to the basic leucine zipper family of DNA binding proteins. Increases in cAMP levels result in the specific phosphorylation of CREB by protein kinase A. Phosphorylated CREB interacts with the transcriptional co-activator CBP (CREB binding protein or p265). It has recently been demonstrated that CREB phosphorylation correlates with light regulated circadian rhythms.

Database

Research Area

DATA IMAGES

Anti-CREB antibody used in Western Blot (WB). GTX27540

GTX27540 WB Image

Anti-CREB is shown to detect CREB-1 present in Raji B cell nuclear extract. Detection occurs using a 1:1,000 dilution of antibody followed by a 1:5,000 dilution of HRP Goat anti Rabbit IgG with visualization via ECL

Anti-CREB antibody used in Western Blot (WB). GTX27540

GTX27540 WB Image

WB analysis of Raji nuclear extract using GTX27540 CREB antibody.
Dilution : 1:1000

REFERENCE

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 589