CREB (phospho Ser121) antibody

Cat No. GTX32165

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human, Mouse, Rat

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Datasheet

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500 - 1:1000
IHC-P	1:100 - 1:200

Not tested in other applications.

Calculated MW

37 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of CREB. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Camp Responsive Element Binding Protein 1,Creb,Creb-1,Creb1

Cellular Localization

Nucleus

Background

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016]

Database

Gene ID: 1385
UniProt: P16220

Research Area

DATA IMAGES

GTX32165 WB Image

WB analysis of colchicine-treated HepG2 (A), mouse kidney (B), rat kidney (C) whole cell lysates using GTX32165 CREB (phospho Ser121) antibody.

GTX32165 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32165 CREB (phospho Ser121) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)