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CREB (phospho Ser129/Ser133) antibody

Orthogonal
Anti-CREB (phospho Ser129/Ser133) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX10564
Anti-CREB (phospho Ser129/Ser133) antibody used in ChIP assay (ChIP assay). GTX10564
Anti-CREB (phospho Ser129/Ser133) antibody used in Western Blot (WB). GTX10564
Anti-CREB (phospho Ser129/Ser133) antibody used in Western Blot (WB). GTX10564

Cat. No. GTX10564

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ChIP assay

Reactivity

 Human, Mouse
Package
50 μl ($399)
See all CREB products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:200-1:2000
ICC/IF 1:100-1:500
ChIP assay 10μl
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Phosphopeptide containing amino acid sequence RRP[pS]YRK, conjugated to KLH.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cAMP responsive element binding protein 1 , CREB , CREB-1

Cellular Localization

Nucleus

Background

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016]

Database

Research Area

DATA IMAGES

Anti-CREB (phospho Ser129/Ser133) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX10564

GTX10564 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX10564 CREB (phospho Ser129/Ser133) antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.25% Trito X-100 for 10 minutes
Dilution : 1:400 for 30 minutes at room temperature

Anti-CREB (phospho Ser129/Ser133) antibody used in ChIP assay (ChIP assay). GTX10564

GTX10564 ChIP assay Image

ChIP analysis of 2 million HeLa cells treated with 50ng/ml of TNF alpha for one hour using GTX10564 CREB (phospho Ser129/Ser133) antibody. Normal Rabbit IgG was used as a negative IP control. The precipitated DNA was detected by PCR with primer set targeting to the promoter of I-kB, YWHAZ gene as positive control target and the inactive GAPDH used as negative control target.

Anti-CREB (phospho Ser129/Ser133) antibody used in Western Blot (WB). GTX10564

GTX10564 WB Image

Orthogonal

WB analysis of 20 μg of NIH/3T3 untreated (lane 1), treated for 10 minutes with 50 ng/ml of PDGF (lane 2), treated for 10 minutes with 200 ng/ml of EGF (lane 3), A549 untreated (lane 4), treated for 10 minutes with 200 ng/ml of EGF (lane 5), A431 (lane 6), SK-N-SH (lane 7), MCF7 (lane 8) and MDA-MB-231 (lane 9) cell lysate using GTX10564 CREB (phospho Ser129/Ser133) antibody.
Dilution : 1:1000

Anti-CREB (phospho Ser129/Ser133) antibody used in Western Blot (WB). GTX10564

GTX10564 WB Image

Orthogonal

WB (peptide competition) analysis of NIH3T3 cells treated with 50ng/mL PDGF for 15 minutes (Lane 2-6) using GTX10564 CREB (phospho Ser129/Ser133) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3), a generic phosphoserine-containing peptide (Lane 4), or the phosphopeptide immunogen (Lane 5) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with lambda phosphatase (Lane 6) eliminates the signal further verifying that the antibody is phospho-specific.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399