GeneTex
United States (US)

CREB (phospho Ser129) antibody

GTX38681_20190129_IHC-P_19012901_138.jpg
GTX38681_20190129_WB_19012901_948.jpg

Cat No. GTX38681

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Specificity/Sensitivity

CREB (phospho-Ser129) antibody detects endogenous levels of CREB only when phosphorylated at serine 129.
PROPERTIES

Form

Liquid

Buffer

PBS (without Mg2+ and Ca2+) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide-KLH. Peptide sequence around phosphorylation site of serine 129 (I-L-S(p)-R-R) derived from Human CREB.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Camp Responsive Element Binding Protein 1,Creb,Creb-1,Creb1

Cellular Localization

Nucleus

Background

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016]

Database

Research Area

DATA IMAGES
GTX38681_20190129_IHC-P_19012901_138.jpg

GTX38681 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX38681 CREB (phospho Ser129) antibody.

GTX38681_20190129_WB_19012901_948.jpg

GTX38681 WB Image

WB analysis of 293 cell lysate using GTX38681 CREB (phospho Ser129) antibody.