GeneTex

  • Country / Location Selection

United States (US)

CREB (phospho Ser133) antibody [HL2776]

Recombinant antibody Orthogonal
Anti-CREB (phospho Ser133) antibody [HL2776] used in IHC (Paraffin sections) (IHC-P). GTX639641
Anti-CREB (phospho Ser133) antibody [HL2776] used in Western Blot (WB). GTX639641
Anti-CREB (phospho Ser133) antibody [HL2776] used in Western Blot (WB). GTX639641

Cat. No. GTX639641

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2776

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all CREB products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser133 of human CREB1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cAMP responsive element binding protein 1 , CREB , CREB-1

Cellular Localization

Nucleus

Background

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016]

Database

Research Area

DATA IMAGES

Anti-CREB (phospho Ser133) antibody [HL2776] used in IHC (Paraffin sections) (IHC-P). GTX639641

GTX639641 IHC-P Image

CREB (phospho Ser133) antibody [HL2776] detects CREB (phospho Ser133) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human oral carcinoma.
CREB (phospho Ser133) stained by CREB (phospho Ser133) antibody [HL2776] (GTX639641) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CREB (phospho Ser133) antibody [HL2776] used in Western Blot (WB). GTX639641

GTX639641 WB Image

Orthogonal

Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CREB (phospho Ser133) antibody [HL2776] (GTX639641) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CREB (phospho Ser133) antibody [HL2776] used in Western Blot (WB). GTX639641

GTX639641 WB Image

Orthogonal

Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CREB (phospho Ser133) antibody [HL2776] (GTX639641) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

There are currently no references for CREB (phospho Ser133) antibody [HL2776] (GTX639641). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for CREB (phospho Ser133) antibody [HL2776] (GTX639641). Be the first to share your experience with this product.
Submit a Review
Package List Price ($)
$ 399
$ 169
Custom Conjugations