Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CREB (phospho Ser133) antibody [HL2776] (GTX639641) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

CREB (phospho Ser133) antibody [HL2776] detects CREB (phospho Ser133) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human oral carcinoma.
CREB (phospho Ser133) stained by CREB (phospho Ser133) antibody [HL2776] (GTX639641) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CREB (phospho Ser133) antibody [HL2776] (GTX639641) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.