CREB (phospho Ser133) antibody

Name: CREB (phospho Ser133) antibody
Brand: GeneTex
SKU: GTX130379
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-CREB (phospho Ser133) antibody used in Western Blot (WB). GTX130379

Cat. No. GTX130379

Reference 5

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all CREB products

View product citations for antibody GTX130379 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000

Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

NIH-3T3 (5 ng/ml PDGF treatment for 15 min) , NIH-3T3 (0.1 μg/ml hFGF2 treatment for 10 min) , PC-12 , 293T

Product Note

This antibody is raised against human CREB phosphorylated at Ser133, and it also may detect the phosphorylated ATF1 protein due to sequence homology.

Predict Reactivity

Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.34 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser133 of human CREB. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886251

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cAMP responsive element binding protein 1 , CREB , CREB-1

Cellular Localization

Nucleus

Background

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq]

Database

Gene ID: 1385 CREB1
UniProt: P16220 CREB1

Research Area

DATA IMAGES

GTX130379 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CREB (phospho Ser133) antibody (GTX130379) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX130379 WB Image

Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CREB (phospho Ser133) antibody (GTX130379) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX130379 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CREB (phospho Ser133) antibody (GTX130379) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX130379 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CREB (phospho Ser133) antibody (GTX130379) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX130379 WB Image

Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CREB (phospho Ser133) antibody (GTX130379) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX130379 WB Image

REFERENCE

Application Reference

YY Hsieh et al. Mol Oncol 2023; Repositioning VU-0365114 as a novel microtubule-destabilizing agent for treating cancer and overcoming drug resistance

Cardile A et al. Int J Mol Sci 2023; 24 (2) Hyperforin Elicits Cytostatic/Cytotoxic Activity in Human Melanoma Cell Lines, Inhibiting Pro-Survival NF-κB, STAT3, AP1 Transcription Factors and the Expression of Functional Proteins Involved in Mitochondrial and Cytosolic Metabolism.

Raineri A et al. Molecules. 2021; 26 (16) : 5027 3,5-Dicaffeoylquinic Acid Lowers 3T3-L1 Mitotic Clonal Expansion and Adipocyte Differentiation by Enhancing Heme Oxygenase-1 Expression.

Application : WB

Reactivity : Mouse

Cao K et al. Aging (Albany NY) 2020; 12 (2) : 1792-1807 The neuroprotective effects of SIRT1 in mice carrying the APP/PS1 double-transgenic mutation and in SH-SY5Y cells over-expressing human APP670/671 may involve elevated levels of α7 nicotinic acetylcholine receptors.

Wu G et al. Epilepsy Research 2017; (Epub) PKC/CREB pathway mediates the expressions of GABAA receptor subunits in cultured hippocampal neurons after low-Mg2+ solution treatment.

Submit a Reference

REVIEW